Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

How should I set cds names when I generate Allele.ctg.table? #19

Closed
LIZW2019 opened this issue Sep 1, 2019 · 2 comments
Closed

How should I set cds names when I generate Allele.ctg.table? #19

LIZW2019 opened this issue Sep 1, 2019 · 2 comments

Comments

@LIZW2019
Copy link

LIZW2019 commented Sep 1, 2019

Hello,

Thank you so much to design such an excellent software to do with HiC assembly! I am a green hand in genome assembly. Now I am going to generate the Allele.ctg.table, but I am confused of the guidance saying "Please modify cds name before running BLAST. The cds name should be same with gene name present in GFF3". Do you mean the ">" line in cds.fasta before blast should be exactly the same as the original gff3 gene name? Is gene ID OK? Can you give an example for me? Thanks a lot!

@tangerzhang
Copy link
Owner

Hi @LIZW2019 ,
If you are working on a diploid genome, you can ignore Allele.ctg.table and use the suggested pipeline:
https://github.com/tangerzhang/ALLHiC/wiki/ALLHiC:-scaffolding-of-a-simple-diploid-genome
If you indeed need Allele.ctg.table for polyploidy assembly and not familiar with coding, you can use a gmap-based method to identify allelic contigs, which is attached here:
#16
This method is simple and does not need annotation of your target genome.

@LIZW2019
Copy link
Author

LIZW2019 commented Sep 2, 2019

Thank you for your reply, it really solved my problem!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants