Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

RNASeq PE illumina data - Poor Reverse read quality? #3

Open
ellango85 opened this issue Nov 29, 2022 · 0 comments
Open

RNASeq PE illumina data - Poor Reverse read quality? #3

ellango85 opened this issue Nov 29, 2022 · 0 comments

Comments

@ellango85
Copy link

While trimming the adaptor and low quality RNA-Seq illumina paired end reads in Trimmomatic, I have got more Forward only survive of about 40 to 50%. This study is for estimate the transcript abundance (DEG) at various condition. How is the possibility to continue further...

  1. USE singleton reads (R1-For only)
    or
  2. Only use both paired (survive) high quality reads (50% of the reads)

Any suggestion, Thanks in Advance

by, Ellango R.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant