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While trimming the adaptor and low quality RNA-Seq illumina paired end reads in Trimmomatic, I have got more Forward only survive of about 40 to 50%. This study is for estimate the transcript abundance (DEG) at various condition. How is the possibility to continue further...
USE singleton reads (R1-For only)
or
Only use both paired (survive) high quality reads (50% of the reads)
Any suggestion, Thanks in Advance
by, Ellango R.
The text was updated successfully, but these errors were encountered:
While trimming the adaptor and low quality RNA-Seq illumina paired end reads in Trimmomatic, I have got more Forward only survive of about 40 to 50%. This study is for estimate the transcript abundance (DEG) at various condition. How is the possibility to continue further...
or
Any suggestion, Thanks in Advance
by, Ellango R.
The text was updated successfully, but these errors were encountered: