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Resulting contig is way longer than expected #162

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kaanb93 opened this issue Feb 15, 2022 · 1 comment
Open

Resulting contig is way longer than expected #162

kaanb93 opened this issue Feb 15, 2022 · 1 comment

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@kaanb93
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kaanb93 commented Feb 15, 2022

Hello,

First of all, thank you very much for such an amazing tool, I've been using it for a while.

On my last assembly, I'm trying to assemble a viral genome with Illumina Novaseq 2x150 reads. Even though I have tried --gsize 10k, I'm ending with only a single contig which is 100k long.

Full code:

shovill --keepfiles --outdir assembly --mincov 2 --minlen 200 --gsize 10k --R1 raw_data/AP_T65_1.fq.gz --R2 raw_data/AP_T65_2.fq.gz

Why does this happen? Is there anything that I could do to limit contig length somehow?

Sincerely,

Kaan

@wvnicholson
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I'm interested in the answer to this question as well and I have a related question which I may post later. It doesn't look there have been any responses to issues for about three years though? Also, no new code for four years,

William

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