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First of all, thank you very much for such an amazing tool, I've been using it for a while.
On my last assembly, I'm trying to assemble a viral genome with Illumina Novaseq 2x150 reads. Even though I have tried --gsize 10k, I'm ending with only a single contig which is 100k long.
I'm interested in the answer to this question as well and I have a related question which I may post later. It doesn't look there have been any responses to issues for about three years though? Also, no new code for four years,
Hello,
First of all, thank you very much for such an amazing tool, I've been using it for a while.
On my last assembly, I'm trying to assemble a viral genome with Illumina Novaseq 2x150 reads. Even though I have tried
--gsize 10k
, I'm ending with only a single contig which is 100k long.Full code:
shovill --keepfiles --outdir assembly --mincov 2 --minlen 200 --gsize 10k --R1 raw_data/AP_T65_1.fq.gz --R2 raw_data/AP_T65_2.fq.gz
Why does this happen? Is there anything that I could do to limit contig length somehow?
Sincerely,
Kaan
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