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Dear developers:
Thank you for the wonderful tool.
Is there any way to know the coverage of reads when using --umi mode?
In umi mode, only read with UB tag is considered, so I think that the coverage is different from that in non-umi mode.
Is my understanding correct?
If so, how do I know the coverage of reads when using umi mode?
One way I came up with is to filter the input bam file to only the reads that have the UB tags in advance, but does this give me the result I want?
Thanks a lot in ahead.
The text was updated successfully, but these errors were encountered:
In order to reduce calls due to sequence error, I would like to use umi mode and further filter by the number of mutant reads. Please tell me if there is a way to know the mutant read count when running umi mode.
Dear developers:
Thank you for the wonderful tool.
Is there any way to know the coverage of reads when using --umi mode?
In umi mode, only read with UB tag is considered, so I think that the coverage is different from that in non-umi mode.
Is my understanding correct?
If so, how do I know the coverage of reads when using umi mode?
One way I came up with is to filter the input bam file to only the reads that have the UB tags in advance, but does this give me the result I want?
Thanks a lot in ahead.
The text was updated successfully, but these errors were encountered: