Skip to content

AllenInstitute/MIES

Repository files navigation

GitHub release (latest by date) Build Main Documentation Signed Installer Coverage pre-commit GitHub contributors

Multichannel Igor Electrophysiology Suite

The Multichannel Igor Electrophysiology Suite (MIES) is a proven sweep based data acquisition software package for intracellular electrophysiology (patch clamp) [1, 2, 3, 4, 5, 6]. It offers top of its class flexibility and robustness for stimulus generation, data acquisition, and analysis.

Highlights

  • Run up to five DACs in parallel for scalable data acquisition.
  • Acquire data on up to eight headstage on a single DAC to probe up to 56 connections at once.
  • Create arbitrarily complex stimulus sets with an intuitive GUI.
  • Export all data, including all of its metadata, into the industry-standard NWBv2-format and read it back in
  • Run custom code during data acquisition for Automatic Experiment Control and real-time adaptability.
  • Automate experimental setup using configuration files for streamlined workflows.
  • Interact with MIES from other programming languages (Python, C++, Javascript, ...) through ZeroMQ.
  • Track and manage your experiments with comprehensive metadata acquisition and browsing tools.
  • Leverage the integrated scripting language for flexible, on-the-fly electrophysiology data evaluation.
  • Ensure compatibility with every earlier version of MIES, protecting your previous work and workflows.

Getting started

Brief visual overview

Slideshow showing the main graphical user interfaces of MIES in Igor Pro

Postsynaptic data analysis

Demonstration of postsynaptic data analysis

Video tutorial of the postsynaptic potential/postsynaptic current data (PSX) analysis module

Required Software

For Data Analysis

  • Windows 10 64-bit or MacOSX 10.10

For Data Acquisition

Supported Hardware

  • Digital to analog converters (DAC):

    • National Instruments:

    • Instrutech/HEKA ITC:

      ITC devices are at the End-Of-Service-Life (EOSL). National Instruments DACs are recommended for MIES users looking for new hardware.

  • Amplifier: Molecular Devices 700B

  • Pressure control (optional):

    • QPV High Resolution Pressure Regulator from Proportion-Air, Inc.; Part name: QPV regulator (custom, see part No), -10 psi to +10 psi; Part no.: QPV1TBNEEN10P10PSGAXLDD
    • controlled through ITC 18/1600 or National Instruments USB 6001

Support statement

The last released version receives fixes for all critical bugs.

Bug reporting

Report a Bug