Can't get the microcopy simulation work

[lipilian]

I try multiple setting combination, change aperture size, change the focal distance ,modify the calibration board, change the sensor objective distance in mm, but I can't see the simulation image based on two microscopy camera model. Any suggestion about these settings? Especially for x60 microscopy.
Thank you,
Best,
Liu Hong

[lipilian]

I attach my microscope simulation blender file here.
MicroScopeSimulation.zip

[Ungetym]

Hi Liu Hong,

I finally found the time to look into the issue and there are two problems:

1. The microscope lenses in Smith's 'Modern Lens Design' are given in a reversed order, i.e. the first entry of the lens table is the lens closest to the sensor whereas I assume, that the first lens is always the closest to the scene. I adjusted the two microscope lens files for the 60x and 100x objectives accordingly and now they are loaded correctly.

2. I think these microscope lenses require an eyepiece counterpart in order to function like a real microscope. The currently available 60x and 100x objectives can only give you clear images if the sensor's diffusor roughness is set close to 0, i.e. when you are basically using an orthographic camera as sensor. I made a sketch of the situation:
   
   I found some eyepieces in Smith's 'Modern Lens Design', however, I am no expert on microscopes, so I am not sure which one would be suited to use with the two already implemented objectives, but in general, it should be simple to include an eyepiece by just attaching the lens table to the microscope lens table in the Lenses folder.

Finally, I prepared a small example setup with Blender 2.91.2 for you (without an eyepiece and with an 'orthographic sensor' instead), so you can check the properties of the microscope objective: https://cloud.rz.uni-kiel.de/index.php/s/SetyoK7yy9qypAP

Best regards,
Tim