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bia-converter

Use

Currently provides a single CLI command convert, which takes as arguments the UUID of an existing ImageRepresentation, and the type of a second representation. Invoked like this:

poetry run bia-converter convert b532b633-9c29-4779-ac2b-7e6c6334ea5f THUMBNAIL

It will determine if the conversion is supported, and, if it is, perform the conversion and upload the result to an S3 location configured by environmental variables.

The code supports conversion of multiple input files to a single output image (e.g. a stack of TIFF files to a single OME-Zarr).

Supported conversions

UPLOADED_BY_SUBMITTER -> INTERACTIVE_DISPLAY (including .ome.zarr.zip) INTERACTIVE_DISPLAY -> STATIC_DISPLAY INTERACTIVE_DISPLAY -> THUMBNAIL

Setup

TBC

TODO:

  • Allow overrides when units are not set correctly
  • More broadly, support passing in conversion options
  • Support routes where we can convert some subtypes of a representation, e.g. we can convert UPLOADED_BY_SUBMITTOR MRC files to PNG
  • Modernise / share the OME-Zarr reading code

zarr2zarr

Examples:

Convert remote Zarr, setting coordinate scales:

poetry run zarr2zarr zarr2zarr https://uk1s3.embassy.ebi.ac.uk/bia-integrator-data/S-BIAD1021/06bc50fb-03ae-4dc5-8a12-89d4f2fcbade/91e29e80-0467-428f-8d96-16cbee80b2fe.ome.zarr/0 local-cache/flower-head1.zarr '{"coordinate_scales": [1.0, 1.0, 13e-6, 13e-6,

13e-6]}'

Convert remote Zarr, transposing T and Z axes, and setting isotropic 2.54 micron voxel size:

poetry run zarr2zarr zarr2zarr https://uk1s3.embassy.ebi.ac.uk/bia-integrator-data/S-BIAD606/73d7bf65-460b-44d7-9b38-d5803c440a28/32f17491-419d-422b-80eb-538567db06e5.ome.zarr/0 local-data/sea-spider2.zarr '{"transpose_axes": [2, 1, 0, 3, 4], "coordinate_scales": [1.0, 1.0, 2.554e-6, 2.554e-6, 2.554e-6]}'

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