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image-utils

Imaging utilities released by the Parker Institute for Cancer Immunotherapy.

Installation

Download from releases.

Usage

$ java -jar image-utils-0.1.0-standalone.jar [command] [args]

If you are working with large images, you may need to increase the maximum heap size.

java -Xmx16G -jar image-utils-0.1.0-standalone.jar [command] [args]

Commands

split-hyperstack

$ java -jar image-utils-0.1.0-standalone.jar split-hyperstack [args]

Splits a tiff hyperstack into individual tiffs. Accepts the following options:

-i or --input Required. Either a tiff file or a directory containing multiple tiff files to be split.

-o or --outpath Optional. A folder to write the output files to. Will create if it does not exist. Defaults to the parent folder of the tiff being split.

-s or --subfolder Optional. If used, generates a folder for each tiff being split and puts the individual tiffs in the subfolder instead of alongside the parent tiff. Tiffs will be named only for the channel and not contain the sample name.

split-tiled

$ java -jar image-utils-0.1.0-standalone.jar split-tiled [args]

Splits a tiled tiff into individual tiffs.

-i or --input Required. Either a tiff file or a directory containing multiple tiff files to be split.

-o or --outpath Optional. A folder to write the output files to. Will create if it does not exist. Defaults to the parent folder of the tiff being split.

-s or --subfolder Optional. If used, generates a folder for each tiff being split and puts the individual tiffs in the subfolder instead of alongside the parent tiff. Tiffs will be named only for the channel and not contain the sample name.

help

Outputs help and usage information.

License

Released under Apache 2 license. See the LICENSE file for details.

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