Metagenomic samples for whole genome sequencing

Description of the ordering procedure for metagenome samples for whole genome sequencing with raw data delivery.

There are 2 ways of placing the order (see instructions below):

• Directly through the Order portal
• Through an order form

Placing the order directly in the Order portal

Providing general information about the order

1. Name the order. If you want to link the new order to an existing ticket in SupportSystem, name the order #[ticket id]
2. Select Metagenome WGS as Type of analysis from the dropdown list
3. Select Raw sequencing data (fastq) as Delivery from the dropdown list
4. Select Customer from the dropdown list
5. Add an optional Comment for the order
6. Press Save

Providing information about the sample

1. Press Add sample
2. Fill in the sample information
3. Special considerations:
   • Application: Be aware that the middle part of the Application tag is determine what library preparation the sample should go through; NXT (Nextera library prep), LIF (Lucigen low input prep), PCF (TruSeq library prep). For guidance click here or contact Clincial Genomics.
4. Add more samples if needed
5. Press Preview and submit the order

Placing the order through an order form

1. Download the order form here.
2. Fill in the order form according to instructions in the "information" tab in the orderform.
3. Submit your order form in the Order portal using the upload orderform field.
4. Check in the Order portal that all information is correct i.e. there are no red markings for any of the samples.
   • For single corrections the easiest way to correct it is directly in the Order portal.
   • If there is a systematic error for several samples, edit the change in the orderform and upload it again.
5. Press Preview and submit the order.