GitHub - GeertsManon/g-qPCR: Recently developed bioinformatic tools - KOMICS and rKOMICS - allowed us to use next-generation sequencing of total cellular DNA extracts as basis to investigate minicircle sequence diversity in 38 animal- and human-infective trypanosome strains. Minicircles were assembled for each Trypanosoma brucei strain independently and grouped into Minicircle Sequence Classes (MSCs) based on a minimum percent identity. This allowed us to identify MSCs that were present in all Tbg1 strains and absent in strains from all other subspecies. We then developed quantitative duplex PCR (qPCR) assays targeting those Tbg1-specific MSCs.

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Recently developed bioinformatic tools - KOMICS and rKOMICS - allowed us to use next-generation sequencing of total cellular DNA extracts as basis to investigate minicircle sequence diversity in 38 animal- and human-infective trypanosome strains. Minicircles were assembled for each Trypanosoma brucei strain independently and grouped into Minicir…

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Name		Name	Last commit message	Last commit date
Latest commit History 2 Commits
CheckMerkers.pbs		CheckMerkers.pbs
JointGenotyping.pbs		JointGenotyping.pbs
Komics.CSBVariations.sh		Komics.CSBVariations.sh
Komics.pbs		Komics.pbs
MappingAndVariantCalling.pbs		MappingAndVariantCalling.pbs
complement.sh		complement.sh
concat.sh		concat.sh
coverage.py		coverage.py
fasta_extend.py		fasta_extend.py
hist.R		hist.R
kDNA.R		kDNA.R
mapping_stats.sh		mapping_stats.sh
qPCR.R		qPCR.R