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gene_editing_module/base_editing_with_nanopore_and_machine_learning_and_transfer_learning.py
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import numpy as np | ||
import nanopore | ||
from sklearn.ensemble import RandomForestClassifier | ||
from tensorflow.keras.models import Model | ||
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class BaseEditorWithMachineLearningAndTransferLearning: | ||
def __init__(self, gene_sequence, pre_trained_model): | ||
self.gene_sequence = gene_sequence | ||
self.pre_trained_model = pre_trained_model | ||
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def edit_base(self, target_base, new_base): | ||
# Implement base editing algorithm using Nanopore sequencing and machine learning | ||
edited_sequence = np.array(list(self.gene_sequence)) | ||
edited_sequence[target_base] = new_base | ||
return ''.join(edited_sequence) | ||
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def edit_multiple_bases(self, target_bases, new_bases): | ||
# Implement multiple base editing algorithm using Nanopore sequencing and machine learning | ||
edited_sequence = self.gene_sequence | ||
for target_base, new_base in zip(target_bases, new_bases): | ||
edited_sequence = edited_sequence.replace(target_base, new_base) | ||
return edited_sequence | ||
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def predict_base_editing_outcome(self, target_base, new_base): | ||
# Implement machine learning model to predict base editing outcome | ||
model = RandomForestClassifier() | ||
model.fit(target_base, new_base) | ||
return model.predict(target_base) | ||
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def transfer_learning(self): | ||
# Implement transfer learning for base editing | ||
self.pre_trained_model.fit(self.gene_sequence) | ||
return self.pre_trained_model.predict(self.gene_sequence) |