Auto Quantification White Area
This ImageJ macro is designed to automatically quantify the white area in an image. It is useful for analyzing images of cells, tissues, and other biological samples.
It will detect and quantify the two largest areas and let you add 2 missed areas or delete 2 incorrectly identified regions.
Installation
- Download and install ImageJ (preferably Fiji distribution)
- Download the auto_quantification_white_area.ijm file from this repository.
- Open ImageJ and drag the auto_quantification_white_area.ijm file into the ImageJ window.
- The macro will now be available in the ImageJ menu under Plugins > Macros > auto_quantification_white_area.
Usage
It should now be open in a new window with easy intructions to follow on the top. Do not change any other lines besides the ones intructed, unless you know what you are doing. The macro will automatically detect the white area in the image, display the results in a table, and save a copy of the image with the quantified area drawn.
License
This software is released under the MIT License.
Acknowledgments
This macro was developed by Nuno Machado. Images for testing and optimization provided by Sara Santos.
If you use it for publications, please refer to Nuno Machado and Champalimaud Foundation, Lisbon, Portugal.
I hope this helps! Let me know if you have any other questions.