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cut off params update #24

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Oct 18, 2023
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cut off params update #24

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Update param.md
hasindu2008 Oct 18, 2023
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Update param.md
hasindu2008 Oct 18, 2023
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29 changes: 20 additions & 9 deletions docs/param.md
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The mysterious default parameters in Guppy.

- Guppy 4.0.11 [dna_r9.4.1_450bps_hac_prom] (qscore cut off default:7)
- Guppy 6.4.2 [dna_r9.4.1_450bps_fast_prom] (qscore cut off - default:8)
- Guppy 6.4.2 [dna_r9.4.1_450bps_hac_prom] (qscore cut off - default:9)
- Guppy 6.4.2 [dna_r9.4.1_450bps_sup_prom] (qscore cut off - default:10)
- Guppy 6.4.2 [dna_r10.4.1_e8.2_400bps_fast_prom] (qscore cut off - default 8)
- Guppy 6.4.2 [dna_r10.4.1_e8.2_400bps_hac_prom] (qscore cut off - default 9)
- Guppy 6.4.2 [dna_r10.4.1_e8.2_400bps_sup] (qscore cut off - default 10)
# The mysterious default parameters in Guppy

## qscore cutoff

The qscore cutoff (that determine pass/fail) in Guppy keeps on changing and there is no easy way to get this. So this is an attempt to document based on what we find.


| Guppy version | Model | default qscore cut off |
|-------- |------ | --- |
| 6.5.7 | dna_r10.4.1_e8.2_400bps_5khz_hac_prom | 9 |
| | | |
| 6.4.2 | dna_r9.4.1_450bps_fast_prom | 8 |
| 6.4.2 | dna_r9.4.1_450bps_hac_prom | 9 |
| 6.4.2 | dna_r9.4.1_450bps_sup_prom | 10 |
| 6.4.2 | dna_r10.4.1_e8.2_400bps_fast_prom | 8 |
| 6.4.2 | dna_r10.4.1_e8.2_400bps_hac_prom | 9 |
| 6.4.2 | dna_r10.4.1_e8.2_400bps_sup | 10 |
| | | |
| 4.0.11 | dna_r9.4.1_450bps_hac_prom | 7 |