Sofie Thijs (sofie.thijs@uhasselt.be)
Here you will find the outline, daily schedule, and powerpoint presentations for the practicum conjugation.
| Time | Activity | Room |
|---|---|---|
| 9h00-9h10 | Biosafety presentation | G2 |
| 9h10-9h50 | Introduction to the practicum | G2 |
| 9h50-12h00 | Genomic DNA extraction | G42 |
| 12h00-13h00 | Lunch Break | |
| 13h00-14h45 | Plasmid DNA extraction | G42 |
| 15h00-16h00 | Gel-electrophoresis of gDNA and plasmid DNA | G42 |
| 16h00-16h15 | Cleaning up your workspace | G42 |
| Time | Activity | Room |
|---|---|---|
| 9h00-9h30 | Results gel electrophoresis and introduction to conjugation | G41 |
| 9h30-11h30 | Conjugation | G41 |
| 12h00-13h00 | Lunch Break | |
| 13h00-14h30 | Read, search and design your selective medium | free work place |
| 14h30-15h30 | Plate conjugation on your selective medium | G41 |
| 15h30-16h45 | Cleaning up your workspace | G41 |
| Time | Activity | Room |
|---|---|---|
| 13h00-13h50 | Microscopy slide preparation and fluorescence microscopy group 1 | G41 + G72 |
| 13h50-14h40 | Plasmid DNA extraction and gel electrophoresis group 1 | G41 |
| 13h50-14h40 | Microscopy slide preparation and fluorescence microscopy group 2 | G41 + G72 |
| 14h40-15h30 | Plasmid DNA extraction and gel electrophoresis group 2 | G41 |
| 14h40-15h30 | Microscopy slide preparation and fluorescence microscopy group 3 | G41 + G72 |
| 15h30-16h20 | Plasmid DNA extraction and gel electrophoresis group 3 | G41 |
| 15h30-16h20 | Microscopy slide preparation and fluorescence microscopy group 4 | G41 + G72 |
| 16h20-17h10 | Plasmid DNA extraction and gel electrophoresis group 4 | G41 |
Follow the report guidelines.
| Time | Activity | Room |
|---|---|---|
| 8h30-9h30 | Feedback on report | online blackboard collaborate |
By the end of the practicum, you should be able to:
-
Conduct, plan and execute an experiment to extract genomic DNA and plasmid DNA from a bacterial strain, and visualize the results on an agarose gel.
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Derive hypotheses for the differences in genome organization (presence of plasmid, yes or no) based on observations of the organisms environment, and lifestyle.
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Explore horizontal gene transfer in bacterial cultures, by transfer of the plasmid encoding for the green-fluorescent protein, between bacteria.
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To develop skills in molecular biology, and sterile techniques for handling bacterial cultures.
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Interpret and compare gel results with fluorescence microscopy images, gfp-expression and the working of a fluorescence microscope.
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Learn how to report against report guidelines.
vincent.stevens@uhasselt.be
leila.paquay@uhasselt.be
sofie.thijs@uhasselt.be