CRISPR-AI Sequence Analyzer

Advanced AI-powered tool for CRISPR-Cas9 sequence analysis and optimization. Features real-time visualization, efficiency prediction, and off-target risk assessment.

Interface Preview

Features

• Interactive GUI with tabbed interface
• Real-time sequence analysis
• Cutting efficiency prediction
• Off-target risk assessment
• Visual result presentation
• Parameter customization
• Comprehensive results reporting

Technical Requirements

• Python 3.8+
• TensorFlow 2.x
• NumPy
• Matplotlib
• Seaborn
• BioPython
• Tkinter

Installation

1. Clone the repository:

git clone https://github.com/yourusername/crispr-ai-analyzer.git
cd crispr-ai-analyzer

crispr-ai-analyzer/
├── src/
│   ├── __init__.py
│   ├── predictor.py
│   ├── visualizer.py
│   └── utils.py
├── tests/
│   ├── __init__.py
│   └── test_predictor.py
├── examples/
│   └── basic_usage.py
├── README.md
├── requirements.txt
└── LICENSE

Create and activate virtual environment: python -m venv venv

Windows

venv\Scripts\activate

Linux/Mac

source venv/bin/activate

Install dependencies: pip install -r requirements.txt Requirements.txt: tensorflow>=2.8.0 numpy>=1.21.0 matplotlib>=3.5.0 seaborn>=0.11.0 biopython>=1.79

Quick Start from crispr_analyzer import CRISPRGui

app = CRISPRGui() app.run()

Basic Sequence Analysis: sequence = "ATCGATCGATCG" app = CRISPRGui() app.sequence_input.insert("1.0", sequence) app.analyze_sequence()

Custom Parameter Settings:

app = CRISPRGui()

app.efficiency_threshold.set(0.8) # Higher efficiency threshold

app.offtarget_tolerance.set(0.2) # Stricter off-target control