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T2T-Assembly

Telomere-to-telomere assmebly workflow

Assembly workflow

pipeline

Quick run:

snakemake -p  --snakefile  pipeline/T2T_Assembly.snakemake.py --configfile pipeline/T2T_Assembly.snakemake.yaml    --jobs 24 

Tools

Most of the tools listed can be installed via Anaconda through searching them in anaconda

The versions listed for thes tools were tested and used in this project.

  • snakemake v5.9.1
  • fastp v0.20.1
  • jellyfish v2.2.10
  • genomescope v2.0
  • hifiasm v0.15.5-r350
  • gfatools v0.4-r214-dirty
  • nanoQC v0.8.1
  • nextDenovo v2.4.0
  • minimap2 v2.17-r941
  • racon v1.4.13
  • mashmap v2.0
  • samtools v1.12
  • bwa v0.7.17-r1188
  • pilon v1.23
  • picard v2.18.29

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