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filterAndTrim #2007
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I'm not sure why you are finding only your forward reads, but I would start by inspecting the filepaths you are providing
No it isn't missing. A few years ago we implemented dereplication "on the fly" in |
Hi, I would like to ask how "filterAndTrim" works. Since I run it with my forward and reward reads but I think it is just doing it by the forward reads. At least in the output I just only find my forward reads. However i am not bekomming any error. And I guess the function is doing this filtering and afterwards filtFs and filtRs should be filtered direct in the folder? - how to check this?
I use the following comands:
cpu = 40
out <- filterAndTrim(cutFs, filtFs, cutRs, filtRs, maxN = 0, maxEE = c(1,1), truncQ = 2, rm.phix=TRUE,
minLen = 100, compress = TRUE, multithread = cpu, verbose = TRUE)
out
Besides I also get a script from a friend where they are doing a dereplication before denoinsing. And indeed in your pipeline says "At this step, the core sample inference algorithm is applied to the dereplicated data." . Is then this step missing?
#dereplication
derepFs <- derepFastq(filtFs, verbose = TRUE)
#denoise reads
dadaFs <- dada(derepFs, err = errF, multithread = cpu, pool = FALSE)
Thank you very much :)
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