import from https://github.com/iseekwonderful/PyPathway on MIT license

pypathway/hotnet/HotNet2.py

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+#!/usr/bin/env python
+
+# Load required modules
+import sys, os, json
+from itertools import product
+
+# Load HotNet2 modules
+from hotnet2 import run as hnrun, hnap, hnio, heat as hnheat, consensus_with_stats, viz as hnviz
+from hotnet2.constants import ITERATION_REPLACEMENT_TOKEN, HN2_INFMAT_NAME
+
+sys.path.append(os.path.normpath(os.path.dirname(os.path.realpath(__file__)) + '/scripts/'))
+import createDendrogram as CD
+
+def get_parser():
+    description = "Helper script for simple runs of generalized HotNet2, including automated"\
+                   "parameter selection."
+    parser = hnap.HotNetArgParser(description=description, fromfile_prefix_chars='@')
+
+    parser.add_argument('-nf', '--network_files', required=True, nargs='*',
+                        help='Path to HDF5 (.h5) file containing influence matrix and edge list.')
+    parser.add_argument('-pnp', '--permuted_network_paths', required=True, default='',
+                        help='Path to influence matrices for permuted networks, one path '\
+                              'per network file. Include ' + ITERATION_REPLACEMENT_TOKEN + ' '\
+                              'in the path to be replaced with the iteration number', nargs='*')
+    parser.add_argument('-hf', '--heat_files', required=True, nargs='*',
+                        help='Path to heat file containing gene names and scores. This can either'\
+                              'be a JSON file created by generateHeat.py, in which case the file'\
+                              'name must end in .json, or a tab-separated file containing a gene'\
+                              'name in the first column and the heat score for that gene in the'\
+                              'second column of each line.')
+    parser.add_argument('-ccs', '--min_cc_size', type=int, default=2,
+                        help='Minimum size connected components that should be returned.')
+    parser.add_argument('-d', '--deltas', nargs='*', type=float, default=[],
+                        help='Delta value(s).')
+    parser.add_argument('-np', '--network_permutations', type=int, default=100,
+                        help='Number of permutations to be used for delta parameter selection.')
+    parser.add_argument('-cp', '--consensus_permutations', type=int, default=0,
+                        help='Number of permutations to be used for consensus statistical significance testing.')
+    parser.add_argument('-hp', '--heat_permutations', type=int, default=100,
+                        help='Number of permutations to be used for statistical significance testing.')
+    parser.add_argument('-o', '--output_directory', required=True, default=None,
+                        help='Output directory. Files results.json, components.txt, and'\
+                              'significance.txt will be generated in subdirectories for each delta.')
+    parser.add_argument('-c', '--num_cores', type=int, default=1,
+                        help='Number of cores to use for running permutation tests in parallel. If'\
+                              '-1, all available cores will be used.')
+    parser.add_argument('-dsf', '--display_score_file',
+                        help='Path to a tab-separated file containing a gene name in the first'\
+                        'column and the display score for that gene in the second column of'\
+                        'each line.')
+    parser.add_argument('-dnf', '--display_name_file',
+                        help='Path to a tab-separated file containing a gene name in the first'\
+                        'column and the display name for that gene in the second column of'\
+                        'each line.')
+    parser.add_argument('--output_hierarchy', default=False, required=False, action='store_true',
+                        help='Output the hierarchical decomposition of the HotNet2 similarity matrix.')
+    parser.add_argument('--verbose', default=1, choices=list(range(5)), type=int, required=False,
+                        help='Set verbosity of output (minimum: 0, maximum: 5).')
+
+    return parser
+
+def run(args):
+    # Load the network and heat files
+    assert( len(args.network_files) == len(args.permuted_network_paths) )
+    networks, graph_map = [], dict()
+    for network_file, pnp in zip(args.network_files, args.permuted_network_paths):
+        infmat, indexToGene, G, network_name = hnio.load_network(network_file, HN2_INFMAT_NAME)
+        graph_map[network_name] = G
+        networks.append( (infmat, indexToGene, G, network_name, pnp) )
+
+    heats, json_heat_map, heat_map, mutation_map, heat_file_map = [], dict(), dict(), dict(), dict()
+    for heat_file in args.heat_files:
+        json_heat = os.path.splitext(heat_file.lower())[1] == '.json'
+        heat, heat_name, mutations = hnio.load_heat_file(heat_file, json_heat)
+        json_heat_map[heat_name] = json_heat
+        heat_map[heat_name] = heat
+        heat_file_map[heat_name] = heat_file
+        mutation_map[heat_name] = mutations
+        heats.append( (heat, heat_name) )
+
+    # Run HotNet2 on each pair of network and heat files
+    if args.verbose > 0:
+        print('* Running HotNet2 in consensus mode...')
+
+    single_runs, consensus, linkers, auto_deltas, consensus_stats = consensus_with_stats(args, networks, heats)
+
+    # Output the single runs
+    if args.verbose > 0:
+        print('* Outputting results to file...')
+
+    params = vars(args)
+    result_dirs = []
+    for (network_name, heat_name, run) in single_runs:
+        # Set up the output directory and record for later
+        output_dir = '%s/%s-%s' % (args.output_directory, network_name.lower(), heat_name.lower())
+        result_dirs.append(output_dir)
+        hnio.setup_output_dir(output_dir)
+
+        # Output to file
+        hnio.output_hotnet2_run(run, params, network_name, heat_map[heat_name], heat_name, heat_file_map[heat_name], json_heat_map[heat_name], output_dir)
+
+        # create the hierarchy if necessary
+        if args.output_hierarchy:
+            hierarchy_out_dir = '{}/hierarchy/'.format(output_dir)
+            if not os.path.isdir(hierarchy_out_dir): os.mkdir(hierarchy_out_dir)
+            CD.createDendrogram( sim, list(index2gene.values()), hierarchy_out_dir, params, verbose=False)
+
+    # Output the consensus
+    hnio.output_consensus(consensus, linkers, auto_deltas, consensus_stats, params, args.output_directory)
+
+    # Create the visualization(s). This has to be after the consensus procedure
+    # is run because we want to default to the auto-selected deltas.
+    if args.verbose > 0:
+        print('* Generating and outputting visualization data...')
+
+    d_score = hnio.load_display_score_tsv(args.display_score_file) if args.display_score_file else None
+    d_name = hnio.load_display_name_tsv(args.display_name_file) if args.display_name_file else dict()
+    for (network_name, heat_name, run), result_dir, auto_delta in zip(single_runs, result_dirs, auto_deltas):
+        snvs, cnas, sampleToType = mutation_map[heat_name]
+        G = graph_map[network_name]
+
+        output = hnviz.generate_viz_json(run, G.edges(), network_name, heat_map[heat_name], snvs, cnas, sampleToType, d_score, d_name)
+
+        with open('{}/viz-data.json'.format(result_dir), 'w') as OUT:
+            output['params'] = dict(consensus=False, network_name=network_name, heat_name=heat_name, auto_delta=format(auto_delta, 'g'))
+            json.dump( output, OUT )
+
+    # Add the consensus visualization
+    snvs, cnas, sampleToType = mutations
+    consensus_ccs = [ d['core'] + d['expansion'] for d in consensus ]
+    consensus_auto_delta = 0
+    results = [[consensus_ccs, consensus_stats, consensus_auto_delta]]
+    with open('{}/consensus/viz-data.json'.format(args.output_directory), 'w') as OUT:
+        output = hnviz.generate_viz_json(results, G.edges(), network_name, heat, snvs, cnas, sampleToType, d_score, d_name)
+        output['params'] = dict(consensus=True, auto_delta=format(consensus_auto_delta, 'g'))
+        json.dump( output, OUT )
+
+if __name__ == "__main__":
+    run(get_parser().parse_args(sys.argv[1:]))

pypathway/hotnet/hotnet2/__init__.py

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+from .constants import *
+from . import delta
+from . import heat
+from . import hnap
+from . import hnio
+from . import hotnet2
+from . import permutations
+from . import run
+from . import stats
+from . import union_find
+from . import viz
+from . import hierarchy
+from .consensus import *
+

pypathway/hotnet/hotnet2/c_ext_src/basic.c

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+//
+//  basic.c
+//  color_coding
+//
+//  Created by Yang Xu on 2017/8/16.
+//  Copyright © 2017年 sheep. All rights reserved.
+//
+
+#include "basic.h"
+#include "data_structure.h"
+#include <string.h>
+#include <stdlib.h>
+
+struct Graph* DiGraphFromMatrix(struct MatrixDes* md) {
+    struct Graph* G = (struct Graph*)malloc(sizeof(struct Graph));
+    G->nodes = (struct Node*)malloc(sizeof(struct Node) * md->length);
+    char * mt = md->matrix;
+    for (int i = 0; i < md->length; i++) {
+        G->nodes[i].degree = 0;
+        G->nodes[i].id = i;
+        for (int j = 0; j < md->length; j++) {
+            if (*(mt + md->length * i + j)) {
+                if (G->nodes[i].degree == 0) {
+                    G->nodes[i].neighbours = (int*)malloc(sizeof(int));
+                    G->nodes[i].neighbours[0] = j;
+                    G->nodes[i].degree++;
+                }else{
+                    G->nodes[i].neighbours = (int*)realloc(G->nodes[i].neighbours,
+                                                           (G->nodes[i].degree + 1) * sizeof(int));
+                    G->nodes[i].neighbours[G->nodes[i].degree] = j;
+                    G->nodes[i].degree++;
+                }
+            }
+        }
+    }
+    G->node_count = md->length;
+    return G;
+}
+
+
+struct SubQueue* stronglyConnectedComponent(struct Graph* G) {
+    // store the result
+    struct SubQueue* result = (struct SubQueue*)malloc(sizeof(struct SubQueue));
+    struct SubQueue* current = result;
+    result->next = NULL;
+    result->queue = NULL;
+    int * preorder = (int *)malloc(sizeof(int) * G->node_count);
+    memset(preorder, 0, sizeof(int) * G->node_count);
+    int * lowlink = (int *)malloc(sizeof(int) * G->node_count);
+    memset(lowlink, 0, sizeof(int) * G->node_count);
+    int * scc_found = (int *)malloc(sizeof(int) * G->node_count);
+    memset(scc_found, 0, sizeof(int) * G->node_count);
+    struct QueueNode* scc_queue = NULL;
+    int i = 0;
+    for (int j = 0; j < G->node_count; j++) {
+        if (G->nodes[j].degree == 0) {
+            // this is an empty node
+            continue;
+        }
+        if (scc_found[G->nodes[j].id]) {
+            // already done
+            continue;
+        }
+        struct QueueNode* queue = initQueue(j);
+        // check if queue is empty while it should be empty
+        while (queue) {
+            int v = queuePeakRight(queue);
+            if (preorder[v] == 0) {
+                i += 1;
+                preorder[v] = i;
+            }
+            int done = 1;
+            // go through beighbours
+            for (int k = 0; k < G->nodes[v].degree; k++) {
+                int nei_id = G->nodes[v].neighbours[k];
+                if (preorder[nei_id] == 0) {
+                    queueAppend(&queue, nei_id);
+                    done = 0;
+                    break;
+                }
+            }
+            if (done == 1) {
+                lowlink[v] = preorder[v];
+                for (int k = 0; k < G->nodes[v].degree; k++) {
+                    int nei_id = G->nodes[v].neighbours[k];
+                    if (scc_found[nei_id] == 0) {
+                        if (preorder[nei_id] > preorder[v]) {
+                            lowlink[v] = lowlink[v] < lowlink[nei_id] ? lowlink[v] : lowlink[nei_id];
+                        }else{
+                            lowlink[v] = lowlink[v] < preorder[nei_id] ? lowlink[v] : preorder[nei_id];
+                        }
+                    }
+                }
+                queuePopRight(&queue);
+                if (lowlink[v] == preorder[v]) {
+                    scc_found[v] = 1;
+                    struct QueueNode* resultQueue = initQueue(v);
+                    // printf("%i, ", v);
+                    while (scc_queue && preorder[queuePeakRight(scc_queue)] > preorder[v]) {
+                        int k = queuePopRight(&scc_queue);
+                        scc_found[k] = 1;
+                        // printf("%i, ", k);
+                        queueAppend(&resultQueue, k);
+                    }
+                    // printf("\n");
+                    if (result->queue == NULL) {
+                        result->queue = resultQueue;
+                    }else{
+                        struct SubQueue* next = (struct SubQueue*)malloc(sizeof(struct SubQueue));
+                        next->queue = resultQueue;
+                        next->next = NULL;
+                        current->next = next;
+                        current=next;
+                    }
+                }else{
+                    queueAppend(&scc_queue, v);
+                }
+            }
+        }
+    }
+    return result;
+}

pypathway/hotnet/hotnet2/c_ext_src/basic.h

@@ -0,0 +1,19 @@
+//
+//  basic.h
+//  color_coding
+//
+//  Created by Yang Xu on 2017/8/16.
+//  Copyright © 2017年 sheep. All rights reserved.
+//
+
+#ifndef basic_h
+#define basic_h
+
+#include <stdio.h>
+#include "data_structure.h"
+
+struct Graph* DiGraphFromMatrix(struct MatrixDes* md);
+
+struct SubQueue* stronglyConnectedComponent(struct Graph* G);
+
+#endif /* basic_h */

pypathway/hotnet/hotnet2/c_ext_src/data_structure.c

@@ -0,0 +1,85 @@
+//
+//  data_structure.c
+//  color_coding
+//
+//  Created by sheep on 2017/8/2.
+//  Copyright © 2017年 sheep. All rights reserved.
+//
+
+#include "data_structure.h"
+#include <stdlib.h>
+
+void queueAppend(struct QueueNode** queue, int val){
+    // if the queue is enpty
+    if (*queue == NULL) {
+        *queue = initQueue(val);
+        return;
+    }
+    struct QueueNode* node = (struct QueueNode*)malloc(sizeof(struct QueueNode));
+    node->value = val;
+    node->next = NULL;
+    node->end = node;
+    struct QueueNode* perious_end = (*queue)->end;
+    node->perious = perious_end;
+    (*queue)->end->next = node;
+    (*queue)->end = node;
+}
+
+int queuePopLeft(struct QueueNode** queue){
+    if ((*queue)->next == NULL) {
+        // only has one node
+        int val = (*queue)->value;
+        *queue = NULL;
+        return val;
+    }
+    struct QueueNode* left = *queue;
+    struct QueueNode* new_left = left->next;
+    new_left->end = left->end;
+    int left_val = left->value;
+    *queue = new_left;
+    return left_val;
+}
+
+int queuePopRight(struct QueueNode** queue){
+    struct QueueNode* right = NULL;
+    if (*queue == NULL) {
+        return -1;
+    }else if ((*queue)->next == NULL) {
+        int val = (*queue)->value;
+        right = *queue;
+        free(right);
+        *queue = NULL;
+        return val;
+    }else{
+        struct QueueNode* cur_end = (*queue)->end;
+        struct QueueNode* new_end = cur_end->perious;
+        (*queue)->end = new_end;
+        new_end->next = NULL;
+        int val = cur_end->value;
+        free(cur_end);
+        return val;
+    }
+}
+
+int queuePeakLeft(struct QueueNode* queue){
+    return queue->value;
+}
+
+int queuePeakRight(struct QueueNode* queue){
+    return queue->end->value;
+}
+
+struct QueueNode* initQueue(int val){
+    struct QueueNode* node = (struct QueueNode*)malloc(sizeof(struct QueueNode));
+    node->next = NULL;
+    node->value = val;
+    node->end = node;
+    node->perious = NULL;
+    return node;
+}
+
+// The structure representation of the graph node
+
+
+
+