Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Data missing when transfroming FASTQ to Adam #1393

Closed
A-Tsai opened this issue Feb 17, 2017 · 0 comments · Fixed by #1394
Closed

Data missing when transfroming FASTQ to Adam #1393

A-Tsai opened this issue Feb 17, 2017 · 0 comments · Fixed by #1394
Labels

Comments

@A-Tsai
Copy link
Contributor

A-Tsai commented Feb 17, 2017

I found some reads are lost when transforming FASTQ to Adam format. There are 500 reads in a FASTQ file, but only 435 reads are available in adam. I use adam flagstat to check the read count and get the following reads. After comparing the result, the last 65 reads are missing. I did use different input file to double check this issue and it happens for all of my testing data.

commands:
adam-submit -- transform -force_load_fastq -print_metrics /test.500.fastq /output
adam-submit -- flagstat /output

Results:
435 + 0 in total (QC-passed reads + QC-failed reads)
0 + 0 primary duplicates
0 + 0 primary duplicates - both read and mate mapped
0 + 0 primary duplicates - only read mapped
0 + 0 primary duplicates - cross chromosome
0 + 0 secondary duplicates
0 + 0 secondary duplicates - both read and mate mapped
0 + 0 secondary duplicates - only read mapped
0 + 0 secondary duplicates - cross chromosome
0 + 0 mapped (0.00%:0.00%)
0 + 0 paired in sequencing
0 + 0 read1
0 + 0 read2
0 + 0 properly paired (0.00%:0.00%)
0 + 0 with itself and mate mapped
0 + 0 singletons (0.00%:0.00%)
0 + 0 with mate mapped to a different chr
0 + 0 with mate mapped to a different chr (mapQ>=5)

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
Projects
None yet
Development

Successfully merging a pull request may close this issue.

2 participants