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This research investigates the impacts of two common fungicide types: copper- and sulphur-based, on the phyllosphere microbiome. DNA sequencing of the 16S gene for bacteria, and the ITS region for fungi allowed us to observe the changes in diversity, abundance, and evenness over the course of three weeks post-treatment.

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brady-welsh/2023_Grapevine_FvP

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Fungicides Vs Phyllosphere Project

Summary

This repository comprises the code and data used for the analysis in the research article:

Welsh, B. L., Eisenhofer, E., Bastian, S. E. P., Kidd, S. P. 2023. "The Effects of Copper- and Sulphur-Based Fungicides on the Leaf Phyllosphere Microbiome of Grapevines." Unpublished.

These notebooks outline the methods (eg., code) that were used for the analyses shown within the publication.

All directories and scripts are numbered in the order in which they were performed.

Data

  • 16S rRNA gene Illumina sequence data (bacteria).

    • The raw sequencing data: BioProject PRJNA1000746
  • ITS gene Illumina sequence data (fungi).

    • The raw sequencing data: BioProject PRJNA1000758

image Photo Credit: Smith's Wine Store

About

This research investigates the impacts of two common fungicide types: copper- and sulphur-based, on the phyllosphere microbiome. DNA sequencing of the 16S gene for bacteria, and the ITS region for fungi allowed us to observe the changes in diversity, abundance, and evenness over the course of three weeks post-treatment.

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