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add e-value threshold to require internal alignments have e-value < 1 #309
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Description
issue: For amplicon sequencing libraries (i.e. sars-cov-2 artic v3 libraries) there were false-positive hits to taxa with e-values in the thousands.
solution: Standard practice is to apply an e-value threshold to ensure that alignments are high-quality. IDseq seeks to maintain high sensitivity, but reduce false-positives with exceptionally high e-values. Thus, a relatively conservative e-value threshold of 1 was implemented to reduce the number of obvious false-positives while still maintaining sensitivity to detect novel organisms.
Version
vX.Y.Z
Tests
Notes