| Environment | Status |
|---|---|
| BioC-release |  |
| BioC-devel |  |
screenCounter quantifies the frequency of barcodes in sequencing data from functional screens, yielding a count matrix that can be subjected to further statistical analyses, e.g., with edgeR and contemporaries. We support single barcode, combinatorial barcode and dual barcode designs, where each construct is assumed to have constant sequence(s) flanking one or more variable regions of known length. The package also provides options for mismatches, strand-specific searching and parallelization via BiocParallel.
For users: check out the documentation at the Bioconductor landing page for installation and usage instructions.
For developers: this R package is just a wrapper around the kaori C++ library that does the heavy lifting. Pull requests for new barcode designs should be made in kaori first, and then propagated back down to screenCounter.