Merge pull request #96 from BioCoderR/master

README.md

@@ -246,6 +246,8 @@ The following items allow for scalable genomic analysis by introducing specializ
 ### Assembly
 - **[SPAdes](https://github.com/ablab/spades)** - SPAdes (St. Petersburg genome assembler) is an assembly toolkit containing various assembly pipelines and the de-facto standard for prokaryotic genome assemblies.
 - **[SKESA](https://github.com/ncbi/SKESA)** - SKESA is a de-novo sequence read assembler for microbial genomes. It uses conservative heuristics and is designed to create breaks at repeat regions in the genome. This leads to excellent sequence quality without significantly compromising contiguity.
+- **[Minimap2](https://github.com/lh3/minimap2)** - Minimap2 is an pairwise aligner for genomic and spliced nucleotide sequences. It can perform the assembly-to-assembly alignment, and works with gzip'd FASTQ, FASTA formats. It also finds overlaps between long-reads.
+- **[D-GENIES](https://dgenies.toulouse.inra.fr/)** - **D**ot plot large **Gen**omes in an **I**nteractive, **E**fficient and **S**imple way. It is an online tool designed to support large genome, compare two genomes, and you can interact with the dot plot to improve the visualisation. It can also be used for extension of minimap2 by uploading the output generated in PAF(Pairwise mApping Format) or MAF(Multiple Alignment File) alignment files to D-GENIES
 
 ### Annotation
 - **[Prokka](https://github.com/tseemann/prokka)** - Prokka: rapid prokaryotic genome annotation. Prokka is one of the most cited annotation command line tools for microbial genome annotations.