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To create an .a2m file, you can perform a multiple sequence alignment of your protein or nucleotide sequences using a software such as Clustal Omega, MAFFT, or MUSCLE, JALVIEW. These software packages can generate .a2m files directly or can convert the output into the A2M format.
I am really new to MSA, would you mind telling me how to get the a2m file that this package required?
Thank you very much.
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