You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
"If fastq_quality_filter complains about invalid quality scores, try removing the -Q33 in the command; Illumina has blessed us with multiple quality score encodings."
Actually this is not accurate. My error is just "Segmentation fault (core dumped)" for not removing "-Q33".
"If you use Illumina 1.5+ without parameter -Q33 you get an error message "fastq_quality_filter: Invalid quality score value..." that clearly indicates pitfall.
But in the opposite case, when you wrongly use -Q33 for reads with Illumina quality format, error messages like
Segmentation fault (core dumped)
or
fastq_quality_filter: bug: got empty array at fastq_quality_filter.c:97
do not give a clue what is going wrong.
"
The text was updated successfully, but these errors were encountered:
https://khmer-protocols.readthedocs.org/en/latest/metagenomics/1-quality.html
In RNASeq doc, it is mentioned.
https://khmer-protocols.readthedocs.org/en/latest/mrnaseq/1-quality.html
"If fastq_quality_filter complains about invalid quality scores, try removing the -Q33 in the command; Illumina has blessed us with multiple quality score encodings."
Actually this is not accurate. My error is just "Segmentation fault (core dumped)" for not removing "-Q33".
see
http://seqanswers.com/forums/showthread.php?t=9357
"If you use Illumina 1.5+ without parameter -Q33 you get an error message "fastq_quality_filter: Invalid quality score value..." that clearly indicates pitfall.
But in the opposite case, when you wrongly use -Q33 for reads with Illumina quality format, error messages like
Segmentation fault (core dumped)
or
fastq_quality_filter: bug: got empty array at fastq_quality_filter.c:97
do not give a clue what is going wrong.
"
The text was updated successfully, but these errors were encountered: