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Co-authored-by: Matt Stone <matthew.stone12@gmail.com>
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nh13 and msto authored Jul 15, 2024
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Expand Up @@ -68,7 +68,7 @@ For a full list of available tools please see the [tools section](http://fulcrum
Below we highlight a few tools that you may find useful.

- Tools for working with Unique Molecular Indexes (UMIs, aka Molecular IDs or Molecular Barcodes):
- Annotating/Extract Umis from read-level data: [`FastqToBam`][fgbio-fastqtobam-link], [`AnnotateBamWithUmis`][fgbio-annotatebamwithumis-link], [`ExtractUmisFromBam`][fgbio-extractumisfrombam-link], and [`CopyUmiFromReadName`][fgbio-copyumifromreadname-link].
- Annotate/Extract Umis from read-level data: [`FastqToBam`][fgbio-fastqtobam-link], [`AnnotateBamWithUmis`][fgbio-annotatebamwithumis-link], [`ExtractUmisFromBam`][fgbio-extractumisfrombam-link], and [`CopyUmiFromReadName`][fgbio-copyumifromreadname-link].
- Manipulate read-level data containing Umis: [`CorrectUmis`][fgbio-correctumis-link], [`GroupReadsByUmi`][fgbio-groupreadsbyumi-link], [`CallMolecularConsensusReads`][fgbio-callmolecularconsensusreads-link], [`CallDuplexConsensusReads`][fgbio-callduplexconsensusreads-link], and [`FilterConsensusReads`][fgbio-filterconsensusreads-link].
- Collect metrics and review consensus reads: [`CollectDuplexSeqMetrics`][fgbio-collectduplexseqmetrics-link] and [`ReviewConsensusVariants`][fgbio-reviewconsensusvariants-link].
- Tools to manipulate read-level data:
Expand All @@ -79,7 +79,7 @@ Below we highlight a few tools that you may find useful.
- Sample pooling QC: [`EstimatePoolingFractions`]: [fgbio-estimatepoolingfractions-link].
- Splice-aware insert size QC for RNA-seq libraries: [`EstimateRnaSeqInsertSize`][fgbio-estimaternaseqinsertsize-link].
- Tools for adding or manipulating alternate contig names:
- Extract from a NCBI Assembly Report: [`CollectAlternateContigNames`][fgbio-collectalternatecontignames-link].
- Extract contig names from an NCBI Assembly Report: [`CollectAlternateContigNames`][fgbio-collectalternatecontignames-link].
- Update contig names in common file formats: [`UpdateFastaContigNames`][fgbio-updatefastacontignames-link], [`UpdateVcfContigNames`][fgbio-updatevcfcontignames-link], [`UpdateGffContigNames`][fgbio-updategffcontignames-link], [`UpdateIntervalListContigNames`][fgbio-updateintervallistcontignames-link], [`UpdateDelimitedFileContigNames`][fgbio-updatedelimitedfilecontignames-link].
- Miscellaneous tools:
- Pick molecular indices (ex. sample barcodes, or molecular indexes): [`PickIlluminaIndices`][fgbio-pickilluminaindices-link] and [`PickLongIndices`][fgbio-picklongindices-link].
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