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Hello! This is an automated update of the following workflow: workflows/microbiome/pathogen-identification/gene-based-pathogen-identification. I created this PR because I think one or more of the component tools are out of date, i.e. there is a newer version available on the ToolShed.
By comparing with the latest versions available on the ToolShed, it seems the following tools are outdated:
toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0 should be updated to toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.2
toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.1+galaxy0 should be updated to toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.5+galaxy1
toolshed.g2.bx.psu.edu/repos/iuc/medaka_consensus_pipeline/medaka_consensus_pipeline/1.7.2+galaxy0 should be updated to toolshed.g2.bx.psu.edu/repos/iuc/medaka_consensus_pipeline/medaka_consensus_pipeline/1.7.2+galaxy1
toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4 should be updated to toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.5+galaxy0
The workflow release number has been updated from 0.1 to 0.2.
If you want to skip this change, close this PR without deleting the branch. It will be reopened if another change is detected.
Any commit from another author than 'planemo-autoupdate' will prevent more auto-updates.
To ignore manual changes and allow autoupdates, delete the branch.
Using nucl database vfdb: 2597 sequences - 2024-Dec-15Processing: nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode10Found 3 genes in nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode10Tip: abricate can also find virulence factors; use --list to see all supported databases.Done.
Using nucl database vfdb: 2597 sequences - 2024-Dec-15Processing: nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode12Found 133 genes in nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode12Tip: have a suggestion for abricate? Tell me at https://github.com/tseemann/abricate/issuesDone.Exception in thread Thread-42 (run_postfork):
Using nucl database ncbi: 5386 sequences - 2024-Dec-15Processing: nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode10Found 0 genes in nanopore_preprocessed_collection_of_all_samples_Spike3bBarcode10Tip: abricate can also find virulence factors; use --list to see all supported databases.Done.
Checking program versionsThis is medaka 1.7.2Program Version Required Pass bcftools 1.15.1 1.11 True bgzip 1.16 1.11 True minimap2 2.24 2.11 True samtools 1.15.1 1.11 True tabix 1.16 1.11 True Aligning basecalls to draftCreating fai index file /tmp/tmpaqgrhj37/job_working_directory/000/9/working/input_assembly.fa.faiCreating mmi index file /tmp/tmpaqgrhj37/job_working_directory/000/9/working/input_assembly.fa.map-ont.mmi[M::mm_idx_gen::0.011*1.09] collected minimizers[M::mm_idx_gen::0.019*1.34] sorted minimizers[M::main::0.022*1.30] loaded/built the index for 13 target sequence(s)[M::mm_idx_stat] kmer size: 15; skip: 10; is_hpc: 0; #seq: 13[M::mm_idx_stat::0.023*1.29] distinct minimizers: 54348 (99.26% are singletons); average occurrences: 1.034; average spacing: 5.248; total length: 294971[M::main] Version: 2.24-r1122[M::main] CMD: minimap2 -I 16G -x map-ont -d /tmp/tmpaqgrhj37/job_working_directory/000/9/working/input_assembly.fa.map-ont.mmi /tmp/tmpaqgrhj37/job_working_directory/000/9/working/input_assembly.fa[M::main] Real time: 0.024 sec; CPU: 0.031 sec; Peak RSS: 0.011 GB[M::main::0.010*1.09] loaded/built the index for 13 target sequence(s)[M::mm_mapopt_update::0.011*1.08] mid_occ = 10[M::mm_idx_stat] kmer size: 15; skip: 10; is_hpc: 0; #seq: 13[M::mm_idx_stat::0.012*1.08] distinct minimizers: 54348 (99.26% are singletons); average occurrences: 1.034; average spacing: 5.248; total length: 294971[M::worker_pipeline::1.557*0.89] mapped 8974 sequences[M::main] Version: 2.24-r1122[M::main] CMD: minimap2 -x map-ont --secondary=no -L --MD -A 2 -B 4 -O 4,24 -E 2,1 -t 1 -a /tmp/tmpaqgrhj37/job_working_directory/000/9/working/input_assembly.fa.map-ont.mmi /tmp/tmpaqgrhj37/files/8/4/8/dataset_84880410-a9fc-43f1-8adb-663a8c06f453.dat[M::main] Real time: 1.559 sec; CPU: 1.392 sec; Peak RSS: 0.134 GBRunning medaka consensus[12:00:52 - Predict] Setting tensorflow inter/intra-op threads to 1/1.[12:00:52 - Predict] Processing region(s): contig_1:0-16886 contig_10:0-19537 contig_12:0-5568 contig_13:0-24846 contig_14:0-1299 contig_15:0-76247 contig_16:0-35171 contig_2:0-41795 contig_3:0-3617 contig_4:0-2172 contig_5:0-7123 contig_8:0-58135 contig_9:0-2575[12:00:52 - Predict] Using model: /usr/local/lib/python3.8/site-packages/medaka/data/r941_min_hac_g507_model.tar.gz.[12:00:52 - Predict] Processing 13 long region(s) with batching.[12:00:53 - MdlStrTF] Model <keras.engine.sequential.Sequential object at 0x7f39cc5edaf0>
[12:00:53 - MdlStrTF] loading weights from /tmp/tmpaqgrhj37/tmp/tmpxbs0pi72/model/variables/variables[12:00:53 - BAMFile] Creating pool of 16 BAM file sets.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_1:0-16886.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_10:0-19537.[12:00:53 - PWorker] Running inference for 0.3M draft bases.[12:00:53 - Feature] Processed contig_1:0.0-16885.0 (median depth 3.0)[12:00:53 - Sampler] Took 0.21s to make features.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_12:0-5568.[12:00:53 - Feature] Processed contig_10:0.0-19536.0 (median depth 4.0)[12:00:53 - Sampler] Took 0.31s to make features.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_13:0-24846.[12:00:53 - Feature] Processed contig_12:0.0-5567.0 (median depth 2.0)[12:00:53 - Sampler] Took 0.18s to make features.[12:00:53 - Sampler] Region contig_12:0.0-5567.0 (5741 positions) is smaller than inference chunk length 10000, quarantining.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_14:0-1299.[12:00:53 - Feature] Processed contig_14:0.0-1298.0 (median depth 4.0)[12:00:53 - Sampler] Took 0.11s to make features.[12:00:53 - Sampler] Region contig_14:0.0-1298.0 (1334 positions) is smaller than inference chunk length 10000, quarantining.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_15:0-76247.[12:00:53 - Feature] Processed contig_13:0.0-24845.0 (median depth 4.0)[12:00:53 - Sampler] Took 0.23s to make features.[12:00:53 - Sampler] Initializing sampler for consensus of region contig_16:0-35171.[12:00:54 - Feature] Processed contig_15:0.0-76246.0 (median depth 4.0)[12:00:54 - Sampler] Took 0.26s to make features.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_2:0-41795.[12:00:54 - Feature] Processed contig_16:0.0-35170.0 (median depth 4.0)[12:00:54 - Sampler] Took 0.21s to make features.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_3:0-3617.[12:00:54 - Feature] Processed contig_3:0.0-3616.0 (median depth 3.0)[12:00:54 - Sampler] Took 0.18s to make features.[12:00:54 - Sampler] Region contig_3:0.0-3616.0 (3707 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_4:0-2172.[12:00:54 - Feature] Processed contig_4:0.0-2171.0 (median depth 5.0)[12:00:54 - Sampler] Took 0.17s to make features.[12:00:54 - Sampler] Region contig_4:0.0-2171.0 (3070 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_5:0-7123.[12:00:54 - Feature] Pileup counts do not span requested region, requested contig_5:0-7123, received 1435-4496.[12:00:54 - Feature] Processed contig_2:0.0-41794.0 (median depth 4.0)[12:00:54 - Sampler] Took 0.44s to make features.[12:00:54 - Feature] Processed contig_5:1435.0-4496.0 (median depth 2.0)[12:00:54 - Feature] Pileup counts do not span requested region, requested contig_5:0-7123, received 4897-6362.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_8:0-58135.[12:00:54 - Feature] Processed contig_5:4897.0-6362.0 (median depth 2.0)[12:00:54 - Feature] Pileup counts do not span requested region, requested contig_5:0-7123, received 6378-6901.[12:00:54 - Feature] Processed contig_5:6378.0-6901.0 (median depth 2.0)[12:00:54 - Sampler] Took 0.20s to make features.[12:00:54 - Sampler] Region contig_5:1435.0-4496.0 (3140 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Sampler] Region contig_5:4897.0-6362.0 (1855 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Sampler] Region contig_5:6378.0-6901.0 (681 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Sampler] Initializing sampler for consensus of region contig_9:0-2575.[12:00:54 - Feature] Processed contig_9:0.0-2574.0 (median depth 1.0)[12:00:54 - Sampler] Took 0.22s to make features.[12:00:54 - Sampler] Region contig_9:0.0-2574.0 (2599 positions) is smaller than inference chunk length 10000, quarantining.[12:00:54 - Feature] Processed contig_8:0.0-58134.0 (median depth 6.0)[12:00:54 - Sampler] Took 0.33s to make features.[12:01:03 - PWorker] Batches in cache: 1.[12:01:03 - PWorker] Processed 1 batches[12:01:03 - PWorker] All done, 8 remainder regions.[12:01:03 - Predict] Processing 8 short region(s).[12:01:03 - MdlStrTF] Model <keras.engine.sequential.Sequential object at 0x7f39c9515d90>
[12:01:03 - MdlStrTF] loading weights from /tmp/tmpaqgrhj37/tmp/tmpxbs0pi72/model/variables/variables[12:01:03 - Sampler] Initializing sampler for consensus of region contig_12:0-5568.[12:01:03 - Sampler] Initializing sampler for consensus of region contig_14:0-1299.[12:01:03 - PWorker] Running inference for 0.0M draft bases.[12:01:04 - Feature] Processed contig_12:0.0-5567.0 (median depth 2.0)[12:01:04 - Sampler] Took 0.11s to make features.[12:01:04 - Feature] Processed contig_14:0.0-1298.0 (median depth 4.0)[12:01:04 - Sampler] Took 0.11s to make features.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_3:0-3617.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_4:0-2172.[12:01:04 - Feature] Processed contig_3:0.0-3616.0 (median depth 3.0)[12:01:04 - Sampler] Took 0.01s to make features.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_5:1435-4497.[12:01:04 - Feature] Processed contig_4:0.0-2171.0 (median depth 5.0)[12:01:04 - Sampler] Took 0.05s to make features.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_5:4897-6363.[12:01:04 - Feature] Processed contig_5:1435.0-4496.0 (median depth 2.0)[12:01:04 - Sampler] Took 0.05s to make features.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_5:6378-6902.[12:01:04 - Feature] Processed contig_5:4897.0-6362.0 (median depth 2.0)[12:01:04 - Sampler] Took 0.04s to make features.[12:01:04 - Feature] Processed contig_5:6378.0-6901.0 (median depth 2.0)[12:01:04 - Sampler] Took 0.02s to make features.[12:01:04 - Sampler] Initializing sampler for consensus of region contig_9:0-2575.[12:01:04 - Feature] Processed contig_9:0.0-2574.0 (median depth 1.0)[12:01:04 - Sampler] Took 0.07s to make features.[12:01:14 - PWorker] Batches in cache: 8.[12:01:14 - PWorker] 22.7% Done (0.0/0.0 Mbases) in 10.5s[12:01:23 - PWorker] Batches in cache: 6.[12:01:29 - PWorker] Batches in cache: 5.[12:01:29 - PWorker] 43.1% Done (0.0/0.0 Mbases) in 25.3s[12:01:34 - PWorker] Batches in cache: 4.[12:01:39 - PWorker] 59.9% Done (0.0/0.0 Mbases) in 35.7s[12:01:44 - PWorker] Batches in cache: 1.[12:01:44 - PWorker] Processed 8 batches[12:01:44 - PWorker] All done, 0 remainder regions.[12:01:44 - Predict] Finished processing all regions.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:46 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:47 - TrimOlap] contig_5:1435.0-4496.0 and contig_5:4897.0-6362.0 cannot be concatenated as there is no overlap and they do not abut.[12:01:47 - TrimOlap] contig_5:4897.0-6362.0 and contig_5:6378.0-6901.0 cannot be concatenated as there is no overlap and they do not abut.[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files./usr/local/lib/python3.8/site-packages/medaka/labels.py:387: RuntimeWarning: divide by zero encountered in log10 q = -10 * np.log10(err)[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files.[12:01:47 - DataIndx] Loaded 1/1 (100.00%) sample files.Polished assembly written to results/consensus.fasta, have a nice day.
Failed to run workflow, at least one job is in [error] state.
history_id
ff7eda6804dad4a7
history_state
error
invocation_id
ff7eda6804dad4a7
invocation_state
scheduled
workflow_id
ff7eda6804dad4a7
gxydevbot
changed the title
Updating workflows/microbiome/pathogen-identification/gene-based-pathogen-identification from 0.1 to 0.2
Updating workflows/microbiome/pathogen-identification/gene-based-pathogen-identification from 0.1 to 0.2
May 19, 2025
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Hello! This is an automated update of the following workflow: workflows/microbiome/pathogen-identification/gene-based-pathogen-identification. I created this PR because I think one or more of the component tools are out of date, i.e. there is a newer version available on the ToolShed.
By comparing with the latest versions available on the ToolShed, it seems the following tools are outdated:
toolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.0should be updated totoolshed.g2.bx.psu.edu/repos/bgruening/split_file_to_collection/split_file_to_collection/0.5.2toolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.1+galaxy0should be updated totoolshed.g2.bx.psu.edu/repos/bgruening/flye/flye/2.9.5+galaxy1toolshed.g2.bx.psu.edu/repos/iuc/medaka_consensus_pipeline/medaka_consensus_pipeline/1.7.2+galaxy0should be updated totoolshed.g2.bx.psu.edu/repos/iuc/medaka_consensus_pipeline/medaka_consensus_pipeline/1.7.2+galaxy1toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4should be updated totoolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/9.5+galaxy0The workflow release number has been updated from 0.1 to 0.2.
If you want to skip this change, close this PR without deleting the branch. It will be reopened if another change is detected.
Any commit from another author than 'planemo-autoupdate' will prevent more auto-updates.
To ignore manual changes and allow autoupdates, delete the branch.