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Title: Add Qualifilter (QC Matrix Extractor, Bioconda-packaged)

Description:
This PR adds Qualifilter, a tool for parsing MultiQC tabular output and generating a summarized QC matrix with key sequencing and mapping metrics such as total reads, coverage, GC content, and contamination. The tool evaluates user-defined QC thresholds and reports Pass/Fail status for each sample.

Key features:

  • Extracts QC metrics from MultiQC .tabular outputs
  • Applies user-defined QC thresholds for total reads, genome coverage, and contamination %
  • Reports Pass/Fail status for each sample
  • Generates outputs in TSV and CSV format
  • Installed via a Bioconda package to simplify dependency management and future updates

Testing:
The tool has passed Planemo lint and Planemo test locally.

Notes:
This PR supersedes #7456
, which was closed because the tool was renamed and moved to a separate repository: Qualifilter GitHub repo
.

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@bgruening bgruening left a comment

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Cool @buhlentozini. Very good quality, just some nitpicks :)

<option value="Unclassified_reads">Unclassified reads</option>
</param>

<param name="total_reads" type="float" value="1000000" label="Minimum total reads" />
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can we add min/max to those parameters to help guide users?

<param name="contam_max" type="float" value="5" label="Maximum contamination %" />
<param name="round" type="integer" value="2" label="Rounding precision" />

<param name="config" type="data" format="yaml" optional="true"
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this file should be described somewhere in more detail I think

@@ -0,0 +1,143 @@
<tool id="qualifilter" name="QualiFilter" version="@TOOL_VERSION@@VERSION_SUFFIX@" profile="21.05">
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Suggested change
<tool id="qualifilter" name="QualiFilter" version="@TOOL_VERSION@@VERSION_SUFFIX@" profile="21.05">
<tool id="qualifilter" name="QualiFilter" version="@TOOL_VERSION@@VERSION_SUFFIX@" profile="@PROFILE@">

@Profile@ Token could also be introduced in the macros.xml. You can assign profile 25.0 is you like.

buhlentozini and others added 7 commits December 4, 2025 12:40
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
Co-authored-by: Saim Momin <64724322+SaimMomin12@users.noreply.github.com>
Co-authored-by: Saim Momin <64724322+SaimMomin12@users.noreply.github.com>
<param name="round" value="2" />
<param name="derive_reads" value="true" />
<output name="qc_matrix_tsv" file="QC_matrix.tsv"/>
<output name="qc_matrix_csv" file="QC_matrix.csv"/>
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The log output is missing in the test. Hence the tests are failing

bgruening and others added 2 commits December 12, 2025 10:59
Co-authored-by: Saim Momin <64724322+SaimMomin12@users.noreply.github.com>
Co-authored-by: Björn Grüning <bjoern@gruenings.eu>
@SaimMomin12 SaimMomin12 merged commit c1d08b0 into galaxyproject:main Dec 15, 2025
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Thanks @buhlentozini for your contribution!

@mvdbeek
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mvdbeek commented Dec 15, 2025

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4 participants