Issue with running Female samples with all chromosomes (22 + XY) even after using --sex-samp F.

[anukrati0306]

Our command .

GangSTR --bam sample1.bam,sample2.bam --bam-samps sample1,sample2, --samp-sex M,F --ref $pathref/Homo_sapiens_assembly38.fasta --regions $pathchr/regions --out $pathvcf/vcf --include-ggl

Facing Error.

[GangSTR-2.5.0] ProgressMeter: Loading read group  sample1 for file sample1.bam
[GangSTR-2.5.0] ProgressMeter: Loading read group  sample2 for file sample2.bam
[GangSTR-2.5.0] ERROR: Not enough reads for sample2 84. Please set insert size distribution manually.

where sample2 is female sample.
and regions bed file consisting of Y chromosome locations as well.