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Two-pass alignment? #28

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mebbert opened this issue Jan 9, 2018 · 0 comments
Open

Two-pass alignment? #28

mebbert opened this issue Jan 9, 2018 · 0 comments

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@mebbert
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mebbert commented Jan 9, 2018

Hi,

I was just reading the paper entitled Two-pass alignment improves novel splice junction quantification, which recommends performing a two-pass alignment (they used STAR) to better identify unknown splice junctions.

Do you have any comments on how this would affect JunctionSeq?

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