geNomad: Identification of mobile genetic elements
geNomad's primary goal is to identify viruses and plasmids in sequencing data (isolates, metagenomes, and metatranscriptomes). It also provides a couple of additional features that can help you in your analysis:
- Taxonomic assignment of viral genomes.
- Identification of viruses integrated in host genomes (proviruses).
- Functional annotation of proteins.
For installation instructions, information about how geNomad works, and a detailed explanation of how to execute it, please check the full documentation: https://portal.nersc.gov/genomad/
geNomad is available as a web app in the NMDC EDGE platform. There you can upload your sequence data, visualize the results in your browser, and download the data to your computer.
If you use geNomad in your work, please consider citing its manuscript:
Identification of mobile genetic elements with geNomad
Camargo, A. P., Roux, S., Schulz, F., Babinski, M., Xu, Y., Hu, B., Chain, P. S. G., Nayfach, S., & Kyrpides, N. C. — Nature Biotechnology (2023), DOI: 10.1038/s41587-023-01953-y.
We recommend users to read the documentation before starting to use geNomad. If you are in a rush, however, you can follow this quick step-by-step example.
First, you need to install geNomad. There's a couple of ways to do that, but here we will use mamba as it will handle all dependencies for us.
# Create an environment for geNomad
mamba create -n genomad -c conda-forge -c bioconda genomad
# Activate the geNomad environment
mamba activate genomad
Alternatively, you can run geNomad using Docker.
# Pull the image
docker pull antoniopcamargo/genomad
# Run the image
docker run --rm -ti -v "$(pwd):/app" antoniopcamargo/genomad
geNomad depends on a database that contains the profiles of the markers that are used to classify sequences, their taxonomic information, their functional annotation, etc. So, you should first download the database to your current directory:
genomad download-database .
The database will be contained within the genomad_db directory.
If you prefer, you can also download the database from Zenodo and extract it manually.
Now you are ready to go! geNomad works by executing a series of modules sequentially (you can find more information about this in the pipeline documentation), but we provide a convenient end-to-end command that will execute the entire pipeline for you in one go.
In this example, we will use an Klebsiella pneumoniae genome (GCF_009025895.1) as input. You can use any FASTA file containing nucleotide sequences as input. geNomad will work for isolate genomes, metagenomes, and metatranscriptomes.
The command to execute geNomad is structured like this:
genomad end-to-end [OPTIONS] INPUT OUTPUT DATABASE
So, to run the full geNomad pipeline (end-to-end command), taking a nucleotide FASTA file (GCF_009025895.1.fna.gz) and the database (genomad_db) as input, we will execute the following command:
genomad end-to-end --cleanup --splits 8 GCF_009025895.1.fna.gz genomad_output genomad_db
The results will be written inside the genomad_output directory.
Three important details about the command above:
- The
--cleanupoption was used to force geNomad to delete intermediate files that were generated during the execution. This will save you some storage space. - The
--splits 8parameter was used here to make it possible to run this example in a notebook. geNomad searches a big database of protein profiles that take up a lot of space in memory. To prevent the execution from failing due to insufficient memory, we can use the--splitsparameter to split the search into chuncks. If you are running geNomad in a big server you might not need to split your search, increasing the execution speed. - Note that the input FASTA file that I used as input was compressed. This is possible because geNomad supports input files compressed as
.gz,.bz2, or.xz.
Note By default, geNomad applies a series of post-classification filters to remove likely false positives. For example, sequences are required to have a plasmid or virus score of at least 0.7 and sequences shorter than 2,500 bp are required to encode at least one hallmark gene. If you want to disable the post-classification filters, add the
--relaxedflag to your command. On the other hand, if you want to be very conservative with your classification, you may use the--conservativeflag. This will make the post-classification filters more aggressive, preventing sequences without strong support from being classified as plasmid or virus. You can check out the default, relaxed, and conservative post-classification filters here.
In this example, the results of geNomad's analysis will be written to the genomad_output directory, which will look like this:
genomad_output
├── GCF_009025895.1_aggregated_classification
├── GCF_009025895.1_aggregated_classification.log
├── GCF_009025895.1_annotate
├── GCF_009025895.1_annotate.log
├── GCF_009025895.1_find_proviruses
├── GCF_009025895.1_find_proviruses.log
├── GCF_009025895.1_marker_classification
├── GCF_009025895.1_marker_classification.log
├── GCF_009025895.1_nn_classification
├── GCF_009025895.1_nn_classification.log
├── GCF_009025895.1_summary
╰── GCF_009025895.1_summary.log
As mentioned above, geNomad works by executing several modules sequentially. Each one of these will produce a log file (<prefix>_<module>.log) and a subdirectory (<prefix>_<module>).
For this example, we will only look at the files within GCF_009025895.1_summary. The <prefix>_summary directory contains files that summarize the results that were generated across the pipeline. If you just want a list of the plasmids and viruses identified in your input, this is what you are looking for.
genomad_output
╰── GCF_009025895.1_summary
├── GCF_009025895.1_plasmid.fna
├── GCF_009025895.1_plasmid_genes.tsv
├── GCF_009025895.1_plasmid_proteins.faa
├── GCF_009025895.1_plasmid_summary.tsv
├── GCF_009025895.1_summary.json
├── GCF_009025895.1_virus.fna
├── GCF_009025895.1_virus_genes.tsv
├── GCF_009025895.1_virus_proteins.faa
╰── GCF_009025895.1_virus_summary.tsv
First, let's look at GCF_009025895.1_virus_summary.tsv:
seq_name length topology coordinates n_genes genetic_code virus_score fdr n_hallmarks marker_enrichment taxonomy
-------------------------------------- ------ ------------------- --------------- ------- ------------ ----------- --- ----------- ----------------- ---------------------------------------------------------------
NZ_CP045015.1|provirus_2885510_2934610 49101 Provirus 2885510-2934610 69 11 0.9776 NA 13 76.0892 Viruses;Duplodnaviria;Heunggongvirae;Uroviricota;Caudoviricetes
NZ_CP045015.1|provirus_3855947_3906705 50759 Provirus 3855947-3906705 79 11 0.9774 NA 14 75.1552 Viruses;Duplodnaviria;Heunggongvirae;Uroviricota;Caudoviricetes
NZ_CP045018.1 51887 No terminal repeats NA 57 11 0.9774 NA 14 67.7749 Viruses;Duplodnaviria;Heunggongvirae;Uroviricota;Caudoviricetes
…
This tabular file lists all the viruses that geNomad found in your input and gives you some convenient information about them. Here's what each column contains:
seq_name: The identifier of the sequence in the input FASTA file. Proviruses will have the following name scheme:<sequence_identifier>|provirus_<start_coordinate>_<end_coordinate>.length: Length of the sequence (or the provirus, in the case of integrated viruses).topology: Topology of the viral sequence. Possible values are:No terminal repeats,DTR(direct terminal repeats),ITR(inverted terminal repeats), orProvirus(viruses integrated in host genomes).coordinates: 1-indexed coordinates of the provirus region within host sequences. Will beNAfor viruses that were not predicted to be integrated.n_genes: Number of genes encoded in the sequence.genetic_code: Predicted genetic code. Possible values are: 11 (standard code for Bacteria and Archaea), 4 (recoded TGA stop codon), or 15 (recoded TAG stop codon).virus_score: A measure of how confident geNomad is that the sequence is a virus. Sequences that have scores close to 1.0 are more likely to be viruses than the ones that have lower scores.fdr: The estimated false discovery rate (FDR) of the classification (that is, the expected proportion of false positives among the sequences up to this row). To estimate FDRs geNomad requires score calibration, which is turned off by default. Therefore, this column will only containNAvalues in this example.n_hallmarks: Number of genes that matched a hallmark geNomad marker. Hallmarks are genes that were previously associated with viral function and their presence is a strong indicative that the sequence is indeed a virus.marker_enrichment: A score that represents the total enrichment of viral markers in the sequence. The value goes as the number of virus markers in the sequence increases, so sequences with multiple markers will have higher score. Chromosome and plasmid markers will reduce the score.taxonomy: Taxonomic assignment of the virus genome. Lineages follow the taxonomy contained in ICTV's VMR number 19.
In our example, geNomad identified several proviruses integrated into the K. pneumoniae genome and one extrachromosomal phage. They were all predicted to use the genetic code 11 and were assigned to the Caudoviricetes class, which contains all the tailed bacteriphages. Since they all have high scores and marker enrichment, we can be confident that these are indeed viruses.
Another important file is GCF_009025895.1_virus_genes.tsv. During its execution, geNomad annotates the genes encoded by the input sequences using a database of chromosome, plasmid, and virus-specific markers. The <prefix>_virus_genes.tsv file summarizes the annotation of the genes encoded by the identified viruses.
gene start end length strand gc_content genetic_code rbs_motif marker evalue bitscore uscg plasmid_hallmark virus_hallmark taxid taxname annotation_conjscan annotation_amr annotation_accessions annotation_description
--------------- ----- ----- ------ ------ ---------- ------------ ----------- ----------------- ---------- -------- ---- ---------------- -------------- ----- -------------- ------------------- -------------- -------------------------------- --------------------------------------------------------------------------------------
NZ_CP045018.1_1 1 399 399 1 0.536 11 None GENOMAD.108715.VP 2.536e-32 123 0 0 1 2561 Caudoviricetes NA NA PF05100;COG4672;TIGR01600 Phage minor tail protein L
NZ_CP045018.1_2 401 1111 711 1 0.568 11 AGGAG GENOMAD.168265.VP 9.279e-47 170 0 0 0 2561 Caudoviricetes NA NA PF14464;COG1310;K21140;TIGR02256 Proteasome lid subunit RPN8/RPN11, contains Jab1/MPN domain metalloenzyme (JAMM) motif
NZ_CP045018.1_3 1143 1493 351 1 0.382 11 AGGAG GENOMAD.147875.VV 1.495e-14 71 0 0 0 2561 Caudoviricetes NA NA COG5633;TIGR03066 NA
NZ_CP045018.1_4 1509 2120 612 1 0.477 11 GGA/GAG/AGG GENOMAD.143103.VP 1.958e-50 179 0 0 1 2561 Caudoviricetes NA NA PF06805;COG4723;TIGR01687 Phage-related protein, tail component
NZ_CP045018.1_5 2183 13516 11334 1 0.566 11 None GENOMAD.159864.VP 1.225e-268 923 0 0 0 2561 Caudoviricetes NA NA PF12421;PF09327 Fibronectin type III protein
NZ_CP045018.1_6 13585 15084 1500 1 0.550 11 AGGAG GENOMAD.195756.VP 2.017e-14 79 0 0 0 2561 Caudoviricetes NA NA NA NA
NZ_CP045018.1_7 15163 16128 966 -1 0.469 11 GGAGG NA NA NA 0 0 0 1 NA NA NA NA NA
…
The columns in this file are:
gene: Identifier of the gene (<sequence_name>_<gene_number>). Usually, gene numbers start with 1 (first gene in the sequence). However, genes encoded by prophages integrated in the middle of the host chromosome may start with a different number, depending on it's position within the chromosome.start: 1-indexed start coordinate of the gene.end: 1-indexed end coordinate of the gene.length: Length of the gene locus (in base pairs).strand: Strand that encodes the gene. Can be 1 (direct strand) or -1 (reverse strand).gc_content: GC content of the gene locus.genetic_code: Predicted genetic code (see details in the explanation of the summary file).rbs_motif: Detected motif of the ribosome-binding site.marker: Best matching geNomad marker. If this gene doesn't match any markers, the value will beNA.evalue: E-value of the alignment between the protein encoded by the gene and the best matching geNomad marker.bitscore: Bitscore of the alignment between the protein encoded by the gene and the best matching geNomad marker.uscg: Whether the marker assigned to this gene corresponds to a universal single-copy gene (UCSG, as defined in BUSCO v5). These genes are expected to be found in chromosomes and are rare in plasmids and viruses. Can be 1 (gene is USCG) or 0 (gene is not USCG).plasmid_hallmark: Whether the marker assigned to this gene represents a plasmid hallmark.virus_hallmark: Whether the marker assigned to this gene represents a virus hallmark.taxid: Taxonomic identifier of the marker assigned to this gene (you can ignore this as it is meant to be used internally by geNomad).taxname: Name of the taxon associated with the assigned geNomad marker. In this example, we can see that the annotated proteins are all characteristic of Caudoviricetes (which is why the provirus was assigned to this class).annotation_conjscan: If the marker that matched the gene is a conjugation-related gene (as defined in CONJscan) this field will show which CONJscan acession was assigned to the marker.annotation_amr: If the marker that matched the gene was annotated with an antimicrobial resistance (AMR) function (as defined in NCBIfam-AMRFinder), this field will show which NCBIfam acession was assigned to the marker.annotation_accessions: Some of the geNomad markers are functionally annotated. This column tells you which entries in Pfam, TIGRFAM, COG, and KEGG were assigned to the marker.annotation_description: A text describing the function assigned to the marker.
In the example above we can see the information of the first seven genes encoded by NZ_CP045018.1. The last entry didn't match any geNomad marker. The first six were all assigned to protein families, some of which are typical of tailed bacteriphages (such as the minor tail protein), reassuring us that these are indeed Caudoviricetes.
One important detail here is that the primary purpose of geNomad's markers is classification. They were designed to be specific to chromosomes, plasmids, or viruses, enabling the distinction of sequences belonging to these classes. Therefore, you should not expect that every single viral gene will be annotated with a geNomad marker. If you want to annotate the genes within your sequences as throughly as possible, you should use databases such as Pfam or COG.
The other two virus-related files within the summary directory are GCF_009025895.1_virus.fna and GCF_009025895.1_virus_proteins.faa. These are FASTA files of the identified virus sequences and their proteins, respectively. Proviruses are automatically excised from the host sequence.
Moving on to plasmids, the data related to their identification can be found in the <prefix>_plasmid_summary.tsv, <prefix>_genes.tsv, <prefix>_plasmid.fna, and <prefix>_plasmid_proteins.faa files. These are mostly very similar to their virus counterparts. The differences in <prefix>_plasmid_summary.tsv (shown below) are the following:
- Virus-specific columns that are in
<prefix>_virus_summary.tsv(coordinatesandtaxonomy) are not present. - The
conjugation_genescolumn lists genes that might be involved in conjugation. It's important to note that the presence of such genes is not sufficient to tell whether a given plasmid is conjugative or mobilizible. If you are interested in identifying conjugative plasmids, we recommend you to analyze the plasmids you identified using geNomad with CONJscan. - The
amr_genescolumn lists genes annotated with antimicrobial resistance function. You can check the specific functions associated with each accession in AMRFinderPlus website.
seq_name length topology n_genes genetic_code plasmid_score fdr n_hallmarks marker_enrichment conjugation_genes amr_genes
------------- ------ ------------------- ------- ------------ ------------- --- ----------- ----------------- ---------------------------------------------------------------------------------------------- -----------------------------------
NZ_CP045020.1 28729 No terminal repeats 36 11 0.9955 NA 6 25.8098 NA NA
NZ_CP045022.1 50635 No terminal repeats 61 11 0.9947 NA 9 46.4657 T_virB1;T_virB3;virb4;T_virB5;T_virB6;T_virB8;T_virB9 NA
NZ_CP045019.1 44850 No terminal repeats 52 11 0.9945 NA 2 28.7110 NA NA
NZ_CP045016.1 82240 No terminal repeats 110 11 0.9939 NA 11 33.4021 T_virB8;T_virB9;F_traF;F_traH;F_traG;T_virB1 NF000225;NF000270;NF012171;NF000052
NZ_CP045017.1 61331 No terminal repeats 76 11 0.9934 NA 15 36.2817 I_trbB;I_trbA;MOBP1;I_traI;I_traK;I_traL;I_traN;I_traO;I_traP;I_traQ;I_traR;traU;I_traW;I_traY NA
NZ_CP045021.1 5251 No terminal repeats 7 11 0.9910 NA 1 1.4225 NA NA