A presentation for engineers with minimal background in biology to understand the structure, mechanisms and clinical development of base editing technology.
A selection of methods and data from Fiumara et. al (Genotoxic effects of base and prime editing in human hematopoietic stem cells 1) is covered in depth, along with a motivation for the experimental design.
- Introduction to gene editing tools
- Cas9 structure and mechanisms
- Base Editors structure and mechanisms
- State of BEs in the clinic
- Defining "Genotoxicity"
- Designing an experiment to evaluate BE behavior
Slides can be found in presentation.md.
Was presented to the Latch engineering team on September 20th, 2023.
Footnotes
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Fiumara, M., Ferrari, S., Omer-Javed, A. et al. Genotoxic effects of base and prime editing in human hematopoietic stem cells. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01915-4 ↩