no header in aln.sam

[maxineliu]

Hi,

I use the command minimap2 -t 30 -2 -I 5g -ax map-pb genome.fa sample_name.fq > aln.sample_name.sam to align sample_name.fa to referencegenome.fa. And use samtools read the sam file with samtools view aln.cy201704.sam | head. Here is the content of the first line:
m64291e_211224_041609/0/42782_42995 16 chr6 116495378 18 4S18M1D4M1D8M1I3M2I26M1D54M5I27M2I14M2I3M2I6M1I8M1I3M3I10M6S * 0 0 GTGCGCCTTTTTTATGGTTGGGTTATAGAGGGAGCCTATGAGATTTCTTGGTATTTTAGACTATTGAGTTGCCTTCCACCCTGCAGATCCCTCGCACTCCCCCGTACTGGATGTAACCCCGTGGTAGGACATGATTTTGCCTCCACCAAACTTCTCACTGTTTTCCTGGTGTATTAATCTCAGCCTCCATGGCGTCCGGTTCCAGTATGGTTT !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! NM:i:32 ms:i:226 AS:i:216 nn:i:0 tp:A:P cm:i:5 s1:i:52 s2:i:0 de:f:0.1122 rl:i:0

It seems that there is no header at all.

Here are the factors that I guess it might result in the consequences. First, the reference genome is 4.3G, I sent 5g for the argument -I, Is 5G not enough big? Besides, the raw data sample_name.for is convert from raw bam file by samtools samtools bam2fq sample_name.subreads.bam > sample_name.fq`, maybe some mistakes were taken in the fq in this step?

Just let me know If there is anything else you want me to provide.

Thanks for help,
Maxine

[maxineliu]

Any clue? Thank you~

[lh3]

Try -I100g.

[maxineliu]

Try -I100g.

Still don't get headers.

The modified command is
minimap2 -t 30 -2 -I 100g -ax map-pb genome.fa sample_name.fq > aln.sample_name.sam

Although it don't complete now, I can read the head of generated sam file by samtools view.

Here is the first line of sam, I guess there is still no header:

m64292e_211225_110414/0/0_9965 0 chr2 248684302 60 512S76M1I18M1I20M1I11M1D5M2I4M1D2M1D10M1D4M1D30M1I8M1D8M1I18M1D15M1D9M1I11M4I22M1D3M1D3M1D12M2D55M1D58M1D76M1I29M1I5M1I4M1I4M1I34M1I41M1D14M1I17M1D7M1D22M1D43M1D32M1D29M1D14M1D11M1I4M1D8M1D30M1D16M1D11M2I13M3I48M1D29M1D34M1D24M1I12M1I40M1I55M1D1M1D22M1I8M1D28M1D3M2I38M1I5M1D2M1D30M1I11M2I41M1D3M1I39M1I7M1D24M1I20M1D3M1I15M1I18M1D1M1D3M1I5M1I5M1I19M1I2M1D8M1I6M4D48M1D22M1I15M3I7M1D25M1D5M1D19M1D20M1I17M1I18M1D3M1D8M1I5M1D83M1D14M6D2M2D15M1D22M34D11M1I40M1I24M1I44M1D6M1D11M2I63M1I4M1D9M1D14M1I3M1I6M1D18M1I11M1I13M1I10M1D28M1I6M1I31M1D12M3I21M1I20M1D1M1D9M1D11M1I8M2I2M1I1M1I10M1I18M1D17M1D13M1I22M2I16M1I5M1D67M1D40M1I18M2I2M1I3M2I32M1I54M1I20M4I14M1I38M3I1M3I8M1D17M1I30M1D8M1I12M1D24M1D35M1I14M1D12M9I18M2I8M2D23M1I7M1D4M1D46M1D13M1D11M1D8M1I8M2I32M1I14M3I56M6I37M1I16M1I16M1D56M1I16M1I27M2I32M2D11M3I2M1I4M1D7M1I10M1I37M1D10M1D29M1I25M1I9M1I3M1D28M2I31M2I13M1D30M1I14M2I8M1D21M1D31M1I17M1D13M2I6M1D18M1I16M1I14M1I27M1I12M1I26M1D13M1I46M1I17M1D13M1D6M1I11M1D27M1I20M1D5M1I10M1D50M1I16M1D11M1I8M1D45M1D22M1D6M4I6M1D5M1I6M1I5M1D75M1I35M1I15M2D12M1D4M1D5M1D8M1D17M1D11M1D10M1D8M1I15M1D24M1I24M1I23M1I11M1I2M1D8M1D44M2I46M1D6M2I28M1I15M2I14M502D50M1I28M1D58M1I17M1I7M1D13M1D3M1I24M1I21M1D8M1I6M2I9M1I6M1D14M1D26M1D13M1I20M1D13M1D7M1I12M1D10M1I61M1D8M1D55M1I11M1I59M1D17M1I8M1D20M1I29M1D38M1I7M1D9M1I14M1D16M2I21M4I13M1D35M1I2M1D93M1D9M1I9M3I25M1I3M1D7M1D19M1D8M1D39M1D26M1D6M1D28M1I10M1D74M1D6M1D35M1I11M1I17M1I12M1I28M1I30M1D28M1I8M1D26M4I3M345I7M4I15M2I1M1I2M1I27M1I12M1D8M1D9M1I7M1I10M1I14M1D34M1I19M2I5M1D34M1D6M1I9M1D45M1D16M1I5M1D4M1I5M1I25M1I8M1I9M1I16M1D13M1I44M1D26M1I45M1I38M1I20M2I45M1I2M1I1M1I17M1D1M1D4M1I2M1I23M1D5M1D41M2I28M1D17M1I18M1I17M1I5M2I4M1I7M1I17M2D2M3I18M5I28M1D8M5I10M1I26M7I27M1D17M1I39M1D20M1D7M2I37M1I19M1I11M1D9M1I38M1D7M1D4M1D6M1I14M1I24M1D7M2I30M1I31M1D35M43I10M1I3M1D23M1D18M1D17M1I6M1I8M1D36M1I7M1D5M1D5M1D3M1D6M1I10M2D49M1I2M1I38M1D10M2I19M1I8M1I3M1I24M1D19M1D93M1D38M1D20M1D16M2I8M5I3M63I34M1I8M1D49M1D16M1I31M2I14M54I3M3I5M4I10M1I2M1I3M38I4M1D17M1D6M1D3M1I12M1D10M1D49M1I22M1I44M1D78M1I10M1D9M1D5M1I24M1I16M1I17M1D34M1I50M1D44M1I34M1I23M1D64M1I11M1D6M2D21M1I36M1D16M1I13M1D5M1D14M1I13M1I10M1I10M * 0 0TAGCGCGCCCCTCCCGATTTTTTCGCAACGCCTAATGCCTCTCCTCTACTCCGCCTGCGGGGTCTTTGCTCGGGGGTCCAACGTTGGGACACGCACCTAAAAGACAATGGGGGCATATCCTAGCCAATATGCCCCCATTGTCTGAGATGAGACAATCCCTTTAAGTGACCAAGTTACTTCCTATCTACTTTTAGTATTTAGAAAATATGGGGGGTTTGGTGGTATTGAAAAGGTTAAAATGAACATAAGGGGCTGGGGATTTATAAAACTAATGTCACGCTGTGATAGCAAGTGGATATTTAGGCCTTAATAGTTTATTCACCAAAAGGCCCTGAGTCAGGAAATTCAGTTATTTGCTTTTCAGTAGCAGACGCTTCATATAAAGGAATTGCACCATGTACTCTATCGACACTTTACATATTGATTGGAATAAGACTGCCACTAAAGGATGGAGTAGTATTTAGGTAATTTTTTAATCCCCAGGACAAGAAACTCATACCATCCCTGACAAGGGAACCCGGTGGTTTTGAAACGTGTTGGATGAACTTTGTCCCTTGGAAGCATCCGTAGGAATGTTGTGATGTCACACCGAACACGCTCCTAAACCTTTTTACCTTTCCCGCTGACGAGGTACTAAAATGCACTACACGTCCTTTTAGTGGATCGTCATAACCAACCTGGAAAGATTAACCCGTTAGGTTGTGTAAGCAGTGGACTAGATCTACAGTAGCCTCCACCACTACTAGCATTATGTTCCAATATACAGCTGTTTTTTTTCGGCTTCCCCTGATTTAGTTCGCTGAGGTTTGTTTGTTTAGCACTGTAGTAGCACTGTTACATATCTAAAGATACTGTATGTGCCCGGGATCTGCACTAACACTACTATATTTGAAAATTGCTCAATTTAATTTGCCTAAATGATTTGGAAATAGTTGGCATGTAAACCATATGAAATGTCACAATTAAAGTGCTTTTAATATATTAGTACAATGCTGTTGATTGTTTTCCATTTCATATCTGGCACTAATGCCTAATGAAGGATGTTTGGTTTATAGTTTGGTCTGTGTAGATTCACTGTAAAAACCAAATCTTTATCTTGTTTTCAGCATATGGGCACTAAGGTAATAATGATTCTGAAAGAGAATTGCTTGAGAAACTATTCATCATCATGAATAACATGCCATTGATTTCCCAGGCTCTCATGGCCTTGCAGACTCTATTCTACACCCGCATCTGGTTCCACAGTAGCCAGAAAGAAATTCTGCTTTGTATATTAATTTTCAGCTCTAAATATGTTATTAGCATTCTGCGATTATTCCATTAATTATCAAGTAACAAACAAACTATCATGGAGTTAATATGCAAGTAACAAATGAAAATGGCAGCACAGGCATAGCCGTTGCTCAGTACAAATATGAAGGAAAGCTATTATTCCCCCTAGCATAATCATCGATACATTTTGACTCGAATATCAGTAATGTAAAATGTTAAAACATAATTATCATTGAACCAATTAACATTAGTTTATATATAAAAAATTCTTTGCAGCAGTTTCAACAAGCCAATCACTTGCTAGCGTTCCACATGTAAATCCTAGAACCTCTGCAGGTGGCCTCTCAGCATGTGTCAGCCCGAAATGTCATGATGCCAATGGCACCAGAATGCTAGAGTCCAAAACCATCTACTAGAGTAAGAGTCACGACGTGACTAAATTTTTGTCAAATCTTTCATCTATCTACCTATCTGTATGTCCTGTCATCATCTACTTTTAACCATTCCCTAATTTATTAACTGCTCCTTGGTTTTTAGGCTGAATATACACATATATGTTGTGTTGCACTTGATTATTTGTTTTATTTAGCAGGTTGGCTAAATCACAGGCCTGGTAACTATGTGCACGATTCCATGTGTGGTGTGGTAAACTGTGCGAAGAAATGACAGAGCCCCAGATTATCAGGAGGGGATCCCCATGGAATTAAGCATTTCATGGGTGGAATCCTTTAGAAGGTTTTGGTCAGAAAGTTTCCCTGCCATTTTTAGTATACCTTGGTCACTGGGCAGAAACATTAGGGTAGTAACTGTATTTAATTGGTCATTTTTTTGGTTGTTGCTATGCTTGGGGGGCAATTTCTTTTGCCATGATGGCAACAAGTATTTATAGTTACTGTTGCATCGAAGGTTGTCAGGGTAGGGAAAGTTTTTTCTTTCCCATATTAAACAGATTTTTGATTGGGTTGTGGGGGAGAGGGGTGGGTATGTGATGTAATTTGGGGGATATATTAAGGGTTTATTATAGTCTGGTACCTTACCTCAACATGGATCTTCAAGTGCACACCTCCCTCCCACCCCTTCTCTTGTCACCGCCTTTGTTTAACCTGGGGGTAAAGGAGCCTATGGCTTGAGCTTATTTTTATTATTTCTATTATTTCTGTTATTGTTGATTATTGTGGTTTAATAGTCTGGTGGGTAATTGAATGTTTAAGTGGTATTGGAATTATTTATGGTGAAGTAAAATAATGGTTTATTTAAGTAAGGTTAACTAATAAACGCAGTGGTCTACAAATTTATCCACAATTATTGTGTATTAATTTAATGGTAATTGTTCTTGCTTGTGACGATGGGTAATGACTATAATCTCATGAGAGTCTCCCTGGTGACGGTTTGCAGTGAAGGTAACAGCGAACTGTTAGACCTAGGAAGAGCCTGCTACTACAAAGTGAGGCATATAGGAGAGGGAGGATGAGAGTCCATCAAGAAGGAGGTTTTGTGGCGGCTTTGAAGGTGCAGTGTTGGCAGCTATTTAATGTGCGTGATCCACATAGGTGCTAAACCCTGAGTAATCTATGAGGTTTAATGTGGATATATTCTGGGATCTGTCATCTGGAGAACTGACTTGTGGTTCCTGTTGAAAAGTGGGGGAGACTGAAGCTGCTGCTGAAGAGTGGTGGGTCACCGACATGGTGAGCTGATGTGAAAGGTCACCTGCCAAGAGTAAACGAGTCTTTGAAAGGAGAGTTTACTTGCCATGTGGGGCCTGCAGTGCAGGTAATGGTGCAGACGTTTGACCTAGCAAAGTAACCAGTTGTTCTCTGGGGCTGATAGAGTTGTGCCATGTAGAAATTTGCACCACCTTGGCTGATGCCAACAAGCTTTTGAACCTCAGACCAAACAAGTGCTAGGCAAAAAAAAAGTCAGCTCAATCACCTTTTTGTTTACCACCTGTCTCACGGAGTGGACTCTGTGCATAGTTAAAATTTCCATCCAGAGCGACCTCCTATTAATAGCAAAATACAAAGTAATTCAGGAACAAGAAGCCCTTATATTATAAAACGTCACTAATGTTAATACACACTACAGCAGAATATATTGTATTCCACCTTGCTGCTGGTGTAATTTATTGCCCCAAAACCGTGCAAAAACTATAACTTGCAAAAAAACACGGCGCAAAGAGGTTCTCTACAAAAAACCTTATGTCTAGCATGTCCGTAAATAAACACGTTCTGACTAATATTCTCACACATACACTTCATACATTTAGTGCATAGGCGTGTGCCAAAATTTAGGTGTGCAAGTCCAAGACACTTTTTTGAGGAGGCATGGTAGTGACATATCACCACCCATAAAATGGAAGTTTCTGAGCTGCTGTACACCAGCAAGGCAACCACACAGTGGGCCGTACCTTGTGCTTCCAACGCGAACTCTGTCCACTGTATAATTTCCAATGCTGGTGGCTGGCAGCTATTTGGTGGGAGGTGCCAGTTGTGGAACCCCACTTATCTGATATTGATGTACTGTTCTTTTGATATGTCATCATTATCTAAGTAACCAGAAAACTCCTTTAAAGGGTTAAATGGTAAACTCACAAGGTACAAGAGTTAGACCTCACCTTAGACACTGAATATTTGCTTCAAAAACCCAGGAATAACGTTATCTGACAAGGCTTAAGGTACCTATGTGATGTTTTTTGATGTCCTCCAGATTTCCTGCTGTTTGTAGGCATTTCATTAAATGTCTCATGTTTGCTTTTTAAAACGATTTAAGGAATTTTGTTCAAGAGGTGAGTGATTTCTCAGTGTGGCAAGCTGTTGGCACCTATCAATGACTATTTACAATTTTTAAAGAGCAACAAAAGCAGTAAAGGTTGGAAATCCACCTACTTACACGCCTCTTGCTAGACTTTGGCGGGATGCAGGTTGTGCAGTTATAGCCATGTTATAAAATATAGATGAAGCAAGCATAGATTAAGCTCCCCCTGAGGCCATTTAGGAATTTAAATTTCATTACTACACTATAGTATATTTCCCTGTAAAGGATATTTTAGCACAAAAATCAACCACATAGCACAAGATCATCAGAAATTTTCCATCTATATTTACATAGTGCTTTATTTATTGCTCGTTTAGCTCAATAGAAAACTCTGCATTTATTCATTTATTTCCCATGAGGCACCTGGAGCAAGAGGATGCGCTGTACAAAAATGTAGACAGATAAAATATTTTTTCCAGTCGTTGTGCACAATTTATTGCAGAAATGTAATCTCCTGACCACAGATGTAAACTGTTTTCCTCTCATCTGGGCCTTGCAGCAACCTCCCCTGAAATGAACAGTGGAGGAAGTGTTTGGAAAACTTGGTTCACAGGCATGCACTTGAGGAATTATAACAGGTCAGTGAATTATGGATGTGTCCTGTCCATGTTCTCTACGACAGCACATATATCCATTGATTTAATTTACACACACGCCTTGTTTTTTACAACTATGGTGGATCCGTGGAAAAAGAGTTGAAGGAACCACGACTTTGGTCCATGGTGTGGACCAAACTCTCCCACTGAAGACCTCTGAGGACCGGGTATCTCCTGTGCCAGTCTTGAATCTTCTGTGCACTGGTGAAATGTACCTAATAATTAGCACATATTTGCCATCTGTGTGTCAAAACGCATATCAATCCAGCCAGGGCTAGCAATAGGCTTGAATTATACTTACTCCAGTTTCTGGCATAAGTTTATAGTCTGACGGAGATGGAGGCTTGTGTCCCCTCCCTATATGCACCAAAACCTTTTCTCCACACTTTAGAAAGAAGCCAGAACCCCAAAAAGTTGCATATTATAGCACAAATAATGGGCACGGACCATAATTTATGACTTTTTCATGCTACCAAAGGGGCATAAAGGAAGGTTGTCTGACTTCTGCAAATGGCATTTAAGCATGTAGAGAAAGTTTTAATACACAGCATTTACTAAGGTATTGTTCTTCTCTATTTTGTCTCATTTGCTGGGTGGATTCAAGGTTTTCATCACATTAGACACAGCTTGTTCCAGGAGTTATGACCACCTTACAAGCCAGCAGTGGTGGTCGTGCTTGCACACTATATGGAAAAAATGCCGACCTGCTCTGGTGCTGGGACTGTGGAGTGGCCCATAGACACCTACCCAGAAGCTTCCTATCCTTGCCACCTCGGACTGCACTGCCTGCGGTCTGGCCTTAACCCCCTGAAACAGGGGGAGAGTATCATCTCCTTTTCACCAGAACAGTGCTTTATAAAGTTAGCAAGCTGTGTGTTTGTAACTTTTAAATGCAACAAAAACTAATGCCTCTTTTTTGCTACCCTCTTCCAACTTTCCAAAGGAAAGCATGACAAGGGTGGTAAAGGGTGGTGGCCAACACCAGAGACAATTTATCTCATTTATACCATTTTTCTGGCACTAATAAAGACAGAAAAGTACGGCAGCTCAGAGGCGGTTGTAGAGTTTTTGTTTAGGCGCATGGACTGCAGGAGGGTGCGCCTAATTTATGAGGCGGCGTACGTAGCTTCCTCCTCATAAATTTAGATGCACCTCCAGTAGCGCAGAGGATTATCAAGACTGATGCAGACAGTGCTGGTCTGATAAATCGCCCCCAAGGAGTGTATAATGGTCCAAACAAGCAGTCACCCAGTGATTCAGCTTATCATCAGGCACACTTCTGCCATAGAAAGAGATGATCCACAGTGCACAACCCCTTTAATATTTCATTATACATCACAGAACTCTTGTAAGATATGCATATAAATACTATCTATATCATACATCCGTATATTAACCATAACACAATAAAGCCTTCCATAGATTTGAGTGAAATATTTGAACTATAGCACATACTAACTGTTCTTAACAATATCAGATCATTTTCCTTTCTATTAAGTCGATTAATGACCCCCTCCATGTCTAGCGTTCACAATAACACACGGGATGCGCCGATTGACTGCACAGTCGCCATTCTTCTGTTCACTGAACTGACTGCCAGCAGGGACGTGCGAGTGCTGAATGAGCCACAGCTACGTCACCAATGACAACCAATACCGCTCTAAAATTATATACCTGACACCCTCTCCCCCTCATTTGATTCTCTCATTTAATTGCAAACATCTTTCTGAGGGTGTATGCTTCATATATCAGTATTATTTTATAGTTCCCTTATTTCAGTTTTTCTTCTGCTTGTGGTCACTTAATCCATGGCTAATACGGCTTTACCCAAAGTGTTTTCCCACTCATAATAACACAGGTCTGACTCTGGAGATCCAAGATATTTAAAATTGCTATTATTTTTATCCGTAGCTAAACAATTTATGTTTACACAAATTTTATTGTTTTAATTTTTGTTAAAAAATTGTGTAAATTTTTTAATTTAACTTCAAAATAAAATTATATTAAAAACAAAAAAATTTTATATAATGTTTTGTATAAAATAAAACCTATAAATTAAAAACATCAGAATTAAAAAGATTTCCCACCATTAAGCATCCCCACCCCCCCCCCCCTTATTAACCACTTTCCTCTATTCCCCCACTCCTTATACCCCCCAGTCCCGGGGGGCATTAAACCCCCATCCCCCGCCCGCACACCAAAACAATTAAATAAACCCCTCCCCCCCCCCCCCCCCCCCCCCCATCCAAAACCCCCCCAATATCCACCCCCCCCCACCCCCCCCCCACACCACGTTGTTTTGTTTTGTATTCAACGTCACGTCTTTCCTTCCGTGCTATTTCCTACCAGTTGAGCCCACACAATGAGAATTCCGTGTTTTTGCTTTTTCTCGCACATGCTAGCAATGGCGAGTTAAAAGTGGACAAGACACTAAACTATGCAGCCCCTATGTTAGGACAGAATCGAGAATGGTAAAGGTCCACTCTAGTATAAGCCTAACCGGCAAAGAACATATTGTGCGTGTGTCTAGTAACAGCTATGAGATAATACAGCCAGCCATTGATCCAGCTGGAGGGGACAGCCAGCAGATTAGCCAATTAAGAATTTTTCTTTATCCCAAAATCCCCGGACATGCCCTAAAACTGTTTACGGACAAATTGAAAACCCTAATGAAATCATAAAGATTAGAAGTAATACCTGTCTATAGCTCAATATACGCTAAGAACTCATCACCGGCATGCACATGTGACTGTAAAATTATGAGAATTACCACCAAAATAATCTAGTCAGGGTACCATCCGCCTCAAAAAATCATGGACCCATCTATTATTTTTCCACAGACACAGGAAACAAAAGATTCTATTTTTACAGACTGTACATAAGCAGCAGGTCGAGCAACATCCCCCCATAAATACTGTAATCCTCAACCTTTGAGTCCCCTGTACCGGAGACGGATTGTCCAATATTCAAAGGTCTTGTGACTGAGGCACTGCATATAAAAAAAACACACCTCGCTCTATGAGCACATGCGTTACTCTGCACCACCCAATGCTCCTGTGCTGACCCCAGACTGATCCCTTATTCGAAATCCCTCCCCCAGGAATTGGCATCTCATCTAGTTCTATGGGGAAGTATAATCATATTCTCTTCTCCCCGAATGTTATGTTTATTGGCCATCCTGCTGAAAAGCAATTTCGATTTTTATTAAGACCAATACTTGATTATCAGTAACTTAAATGGTGTGTGGTAGAAGCACTGTGACATTTGGTATGTGATGGCCGATGCATTATAATTGGTACCAAATATGTGGGAATTGTTTTCTTTAATGCCATGAGAATACCATTCTATCATTGGGTTACTACACGAATATAATTTGATTTACAACACCTCCAGTTGCCTCCATTTTCTGTATTGTTGTTTTTTGGGAGTCTTTTTGTTATATGTGGTCCCCTGATGAACTAACCTAGAGACGGTCAGAGAAAACGCATCGGGACAAAAGTTTGCATACCTGGGACATATGTATGTCCACACTGGTGATCAAAACGTGGACGCCTCTGGTTTTATTTGGCAGCATATACGTATTATGTGCGTTATTTTTGTTGTAACTTTCTTATTTTCTGATACATCATGGACTTTGACATGACATTGCTATTGGACTGAAGTAATACTAGGTCCCTTCCTCGGTGGCTGTATCTATCACTTGTCGGGACATATAGGGCCTATAGGAGGTTCCTGACAGGAGGATTGCCCCCTATCGGGCGGCCATTCCATTTTGCGAGATGGGACCTATAGTTTTCATAGGCAATGTGAGGTGGTCAATTTTTCCTTTCTGTCCTGCCCTACCTCATTATTAATTGCTTTTTATGTACACAAATTGGGTTGAATTTCTTTATGATCATCAGTAAAGGTTACGTTTTACACGGTGAAGGTATTCTCTGTGATGACCTGCTTCTTTTAAATTGGATCCAACATTTTCCAGAAATACATCCACAAAAAGCAGATATAGTGAAACTGCATTTTTTGGAATAAAAAAAACTCACATGACTATAGACAGAACTGATCACATTACCCTTTAATTTGTAGTTATTTTGGGACCAGAGTGCGAACTTTTACAATAAATACGGAATATAAGGATTAAGTATTTAAAGCATCAGATATTATGTCCCTGGTTTAAAAAGTATGGATCCTTTAATAATACCTTCTGCACTCCAGCTGCAGTAAAACACTACAACTCCAGCATACAAACTGGATTGGCTGTTTTTGTAACGTCAAAAGAAACGAAAGGAGGATTCCTAGGTGCATAGTTTCAGAACAGAAGGAGTGCCGGAGGTTGCTGATCCCTGGTTAATGGTGCCTGAACCTTTGTTATAAACCAACAAAGAGCAAAAGCACTACGCAGAGACAAAAACTGCCTGCCAATCTTCAACCATCACATGCCCTTTTTTTCGAGTGTATGGAGTATGAAAATCTAACTCCACATATGCTCGAAAAGCCTGGAGCAAGCGATGAAAGACATTTTTCTAAAATATGCATTTCTCTGCTTTATGGCAGGTAGTGATTTTATGCATGGTAGTGATACCCACAAAATAGTATTATGTAGCATTTTCCATATGTCTACTTGATGTTGGCATCATTTTGTAAAATGTCATTACATTTTTTTAGAAATGTTTGAAGGCTTAGAATTTTAGAAGCAATTTTTCAAATTTAAACAAAATTTCCAAAACTATTTTCAAGCACCAATTCAGTTATAAAGTGATTTTGAGAGGCTTACGTAATGGAAAGCACTCCATAAATGACCAATTTTAATATTAACACCCTTTAAATTATTTAACACTAGAGGTTGCAAACTTTGTTTAACCCTTGATGTGTTCACAAGAGTTAATGGAAAATAGAGGTGAAATTCCAAAAATGTCATTTTTTTTTTAGACTTTTTATGTTAATTTATTTTTTATTGTACATAGCAAGGGTTAACAGCAAAATAAACCTCAATATACATAACGCCTGATTATTCAGAAATACCCCATATGTGTGGGTAAAACTGCTGTAGGGGCACATAGCGTGGTCAGAAGGAAAGGAGCACCATATGTATTTTGTATTTAAAGAGACCCTGATGTACCCCTACAAGTGGAAACCCCAAAAATACCCCATTTTGGAAACTACACCCCCTCAAAGAATATATTAAGGAGTGTACTGAGCACTAGACCCATAAGCGTTGTAACGGAATTTGGAACACTGGCTGTGAAAATGAAAAAGTTTAATTTGCTTCCAATAACAATGTTGCTT !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! NM:i:2044 ms:i:11818 AS:i:10567 nn:i:0 tp:A:P cm:i:249 s1:i:3384 s2:i:99 de:f:0.0946 rl:i:55

[jmarshall]

Recently crossposted to biostars, where the issue has been solved (and is unrelated to minimap2):

And use samtools read the sam file with samtools view aln.cy201704.sam | head

If you want to view the headers, you need samtools view -h …. Or — it's a SAM file, so just look at it directly with more.

[lh3]

Oh, yes. Should have realized this earlier.