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This program takes a directory full of nascent fastq files as inputs. It outputs bams, bedgraphs, and tdfs. to run: python create_scipts_to_map_on_fiji_argparse.py <indir> <outdir> <genome> <email> for example: python create_scipts_to_map_on_fiji_argparse.py /scratch/Users/allenma/pro/fastqfiles/ /scratch/Users/allenma/pro/ hg19 Mary.A.Allen@colorado.edu important: You must git clone this somewhere on /scratch/. Additionaly, Both your indir and outdir must be on scratch. More information: This script will make a slurm script for processing GRO-seq data and submit it to the queue on fiji. Buy defalut the program will map the reads using bowtie2, then convert the sam to a sorted bam file, then create bedgraphs, then make tdf files which can be used in the program IGV. positional arguments: indir Directory with fastq or bam files. Must be located on /scratch/. outdir Directory files will be output into. The program will create this direcoty if it does not exist. This direcory must be located on /scratch/. genome Which genome do you wish to map too? Allowed values are hg19, dm3, mm10, email Email address is required. If you mistype your email then IT gets your emails. Please don't do that. optional arguments: -h, --help show this help message and exit -f {True,False}, --flipreads {True,False} -q {True,False}, --checkquality {True,False} -t {True,False}, --trimgalore {True,False} -m {True,False}, --fastqtosam {True,False} -s2b {True,False}, --samtobam {True,False} -b2sb {True,False}, --bamtosortedbam {True,False} -ib {True,False}, --sortedbamtobai {True,False} -bg {True,False}, --sortedbamtobedgraph {True,False} -c {True,False}, --readcountcorrectbedgraph {True,False} -igv {True,False}, --igvcreate {True,False}
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