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* WIP * WIP * fixed examples and minor problem with SingleInputSelector
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LOCUS CU329670 4538 bp DNA UNK 01-JAN-1980 | ||
DEFINITION . | ||
ACCESSION SPAPB1A10.09 | ||
VERSION SPAPB1A10.09 | ||
KEYWORDS . | ||
SOURCE . | ||
ORGANISM . | ||
. | ||
FEATURES Location/Qualifiers | ||
3'UTR 394..676 | ||
/systematic_id="SPAPB1A10.08" | ||
/db_xref="PMID:21511999" | ||
5'UTR 1001..1173 | ||
/systematic_id="SPAPB1A10.09" | ||
/db_xref="PMID:30566651" | ||
/feature_source="evidence=ECO:0000309; source=SO:0001238; | ||
condition=FYECO:0000126; during=GO:0072690; score=146.757; | ||
db_xref=PMID:30566651" | ||
CDS join(1174..1597,1645..3416) | ||
/colour="2" | ||
/primary_name="ase1" | ||
/product="antiparallel microtubule cross-linking factor | ||
Ase1" | ||
/systematic_id="SPAPB1A10.09" | ||
/controlled_curation="term=modification, phosphorylated; | ||
db_xref=PMID:19547744; evidence=IDA; cv=pt_mod; | ||
date=20100606" | ||
/controlled_curation="term=orthologous to S. cerevisiae | ||
YOR058C; date=19700101" | ||
/controlled_curation="term=species distribution, conserved | ||
in eukaryotes; date=20081110" | ||
/controlled_curation="term=species distribution, conserved | ||
in metazoa; date=20081110" | ||
/controlled_curation="term=species distribution, conserved | ||
in vertebrates; date=20081110" | ||
/controlled_curation="term=species distribution, | ||
predominantly single copy (one to one); date=20081110" | ||
/controlled_curation="term=species distribution, conserved | ||
in fungi; date=20081110" | ||
/controlled_curation="term=species distribution, conserved | ||
in eukaryotes only; date=20081110" | ||
/translation="MQTVMMDDIQSTDSIAEKDNHSNNESNFTWKAFREQVEKHFSKIE | ||
RLHQVLGTDGDNSSLFELFTTAMNAQLHEMEQCQKKLEDDCQQRIDSIRFLVSSLKLTD | ||
DTSSLKIESPLIQCLNRLSMVEGQYMAQYDQKLSTIKEMYHKLESYCNRLGSPFVLPDF | ||
ENSFLSDVSDAFTESLRGRINEAEKEIDARLEVINSFEEEILGLWSELGVEPADVPQYE | ||
QLLESHTNRPNDVYVTQELIDQLCKQKEVFSAEKEKRSDHLKSIQSEVSNLWNKLQVSP | ||
NEQSQFGDSSNINQENISLWETELEKLHQLKKEHLPIFLEDCRQQILQLWDSLFYSEEQ | ||
RKSFTPMYEDIITEQVLTAHENYIKQLEAEVSANKSFLSLINRYASLIEGKKELEASSN | ||
DASRLTQRGRRDPGLLLREEKIRKRLSRELPKVQSLLIPEITAWEERNGRTFLFYDEPL | ||
LKICQEATQPKSLYRSASAAANRPKTATTTDSVNRTPSQRGRVAVPSTPSVRSASRAMT | ||
SPRTPLPRVKNTQNPSRSISAEPPSATSTANRRHPTANRIDINARLNSASRSRSANMIR | ||
QGANGSDSNMSSSPVSGNSNTPFNKFPNSVSRNTHFESKSPHPNYSRTPHETYSKASSK | ||
NVPLSPPKQRVVNEHALNIMSEKLQRTNLKEQTPEMDIENSSQNLPFSPMKISPIRASP | ||
VKTIPSSPSPTTNIFSAPLNNITNCTPMEDEWGEEGF*" | ||
3'UTR 3417..3538 | ||
/systematic_id="SPAPB1A10.09" | ||
/db_xref="PMID:21511999" | ||
3'UTR complement(3510..3690) | ||
/systematic_id="SPAPB1A10.10c" | ||
/db_xref="PMID:21511999" | ||
ORIGIN | ||
1 atcatcagac gtgtatttca caagccagaa gtgcatttgg atccaagtgc ctccatttta | ||
61 aatctctcat cttcgcatgg cgaaagcaac ctgacaaatg gtttgctttg tcaaaatttc | ||
121 aagctttttc aggatgattg gttgatggag gattgtgcgc cagatgccaa tttcactttg | ||
181 tacaccccgc ttcaaccctg ggaaaagcga agtgtgaaac ctgaaatcag acgtcctcga | ||
241 ttaaatccta attttttccg agtatttgtt ttagaagctc aaatgcgacg agctggaaag | ||
301 ctatcagcaa acactgctgg ccgagcccag ttaatttacc tcccaaagcc tgccgttacc | ||
361 ttctccacta gccctttgca tgttgaattg taaaaattta acgcatgact tatatacatt | ||
421 tgcattcttc caagctggtt atatttattt tcattttttt ctcacccaat acttttttat | ||
481 ccctactgtc tttatggaca atcgactcac aattgtttct ttttgttgta tatgattttt | ||
541 tttttaaagg aaatgggttt cgcgatactg ggttgaatcc caattgcggt taatattaca | ||
601 taaaataatt ctcccatagt cctagatcct gtctttgaat atgagcaaat aaaagaattg | ||
661 aacaaatcat gaatgctttt ctctcttaga tgatattttg tatgcataag tctaattata | ||
721 ttgattacga taagacttaa aaagtaagcc tttgtatcct tttaagcagt atttgaattt | ||
781 tcttgtatca tattttaggt agagcaaaag ataccagttt gtagaacttt atgtgcttcc | ||
841 ttacattggt atatttcagg cacataaata ttcttcaact tacaattcta agtattttgt | ||
901 ttatactaaa aggagctgaa taacgtttat acagtgctga cattgaaatc tatttgcttt | ||
961 ctttggaata taagcgcatg ctgagttact ttcgcaggcc aagccatatc caaccaccat | ||
1021 ttttgtgcca agcttttatg caaggttaat tccttgtact gcttgttatg ttataatata | ||
1081 tcaacatctt aacagttttc atatcttcct ttatattcta ttaattgaat ttcaaacatc | ||
1141 gttttattga gctcatttac atcaaccggt tcaatgcaaa cagtaatgat ggatgacatt | ||
1201 caaagcactg attctattgc tgaaaaagat aatcactcta ataatgaatc taactttact | ||
1261 tggaaagcgt ttcgtgaaca agtggaaaag catttttcta aaattgaaag gcttcaccaa | ||
1321 gtccttggaa cagatggaga caattcatca ttatttgagt tgtttacaac ggcaatgaat | ||
1381 gcccagcttc atgaaatgga acagtgccag aaaaaacttg aagatgactg tcagcaaaga | ||
1441 attgattcaa tcagattttt ggtttcctca ttaaagttaa cggatgatac ttctagtctc | ||
1501 aaaattgagt ctcctttaat tcagtgtttg aatcgtttgt caatggtaga aggacaatat | ||
1561 atggcacagt atgatcaaaa gttaagtacg attaaaggta tgtaatcgtc tttaatttag | ||
1621 acttgtgttt taactgatgt atagaaatgt atcacaaatt ggagtcatat tgtaaccgct | ||
1681 taggaagtcc gttcgtttta cctgattttg agaattcatt tttatctgat gtatccgatg | ||
1741 cttttactga atctttgaga ggacgcatca acgaagccga aaaggagatt gatgcgagat | ||
1801 tagaggttat taattccttt gaagaagaaa ttttgggttt gtggtctgaa ctcggtgttg | ||
1861 agcccgctga tgttccacaa tacgaacaat tgcttgaatc ccatactaat cgaccaaatg | ||
1921 atgtttatgt tactcaagaa cttatcgacc aactttgcaa gcaaaaagaa gttttttccg | ||
1981 ctgaaaaaga aaagagaagt gatcatttaa aaagtataca atcagaagtt agcaacttgt | ||
2041 ggaataagct tcaagtttct cccaatgaac aaagtcaatt tggcgattca tcaaacatta | ||
2101 atcaagaaaa tatttcatta tgggaaactg aacttgaaaa acttcatcag ttaaaaaagg | ||
2161 agcatttacc cattttttta gaagactgtc gtcaacaaat tcttcagctt tgggattctc | ||
2221 tgttttattc agaagaacaa agaaagtcct ttacacctat gtatgaagac attattacag | ||
2281 agcaggttct tacggcccat gaaaactata taaagcaact agaggccgaa gtttctgcta | ||
2341 ataagtcctt tttaagctta attaatcgct atgcctcttt aatagaagga aagaaagagc | ||
2401 ttgaagctag ttctaatgat gcctctcgtc taacacaacg gggacgccgg gacccaggtt | ||
2461 tacttctacg tgaagagaaa atccgtaagc gactttctag agaacttcct aaggttcagt | ||
2521 cgctgcttat accagagatt acagcatggg aagaaagaaa tggaaggacg ttcctttttt | ||
2581 atgatgaacc acttctcaag atttgccaag aggccactca accaaaatca ttatatagaa | ||
2641 gtgcaagtgc tgccgcaaac cgcccgaaaa cagcaactac aacggactct gttaatagaa | ||
2701 caccttctca acgagggcgt gtagctgtac cttcaacacc aagtgttagg tccgcttctc | ||
2761 gagctatgac gagtccaagg acaccgcttc ctagagtaaa aaacactcaa aatccaagtc | ||
2821 gttccattag tgcagaaccg ccatcagcaa ccagtaccgc caatagaaga caccccactg | ||
2881 ctaatcgaat tgatataaac gctagattaa acagtgctag tcggtctcga agcgcgaaca | ||
2941 tgataagaca aggggcaaat ggtagtgaca gcaatatgtc ttcttcaccc gtttctggaa | ||
3001 attccaatac cccttttaac aagtttccaa attctgtatc tcgcaataca cattttgaat | ||
3061 ccaagtcacc gcacccaaat tactctcgaa ctcctcatga aacgtattca aaggcttcat | ||
3121 ctaagaacgt cccattaagt cctccaaagc agcgtgtagt taatgaacac gctttaaata | ||
3181 ttatgtcgga aaaattgcaa agaactaatc tgaaagaaca aacacccgag atggacattg | ||
3241 aaaacagctc gcagaacctt cctttttctc ctatgaagat atcccccata agagcatcac | ||
3301 ccgtaaagac aattccatca tcaccgtccc ccactaccaa cattttttct gctccactca | ||
3361 acaatattac aaattgtaca ccgatggagg atgaatgggg agaagaaggc ttttaagctt | ||
3421 cttatttacc taatcgatca aatttaaata tacatatttt tgcatatgaa tacagcatat | ||
3481 agataattca taaaagttta ttaactgagg tcataattaa aagactattt acacctaaaa | ||
3541 aaaaacgtgt atcaatagag ggaaaagaga agaattaaga acagaaagta accatagttt | ||
3601 tgttaaaata gcaatgtaaa aaaatattat gaaaagaaaa cgtatagcac attttgaaat | ||
3661 gtaaaagaat ctgagagagc gtgtgaatat ctagcaatta caagaagatg tattattcaa | ||
3721 aggctttgaa agaagcaaag gttcagagaa gtcattaaca aagtcatctc tcgagctttc | ||
3781 attttctaaa gctaaacgac tgactgtttc gaaaaggtca gtaacgtttg tattttcttt | ||
3841 tgcactagct tcaaaatgaa tcatatttga tccatgtttg gatttgcaat agtcaagagc | ||
3901 tcgatgaaga gatacggctc gtttagacgc gtctttgtcg atttgatttc caacgataat | ||
3961 gaaagggaat gcacattcat cttgtgaagt ttgatataaa aattcttgcc tccagttttc | ||
4021 tactgagtca aaagacttcg agttattcac attataaaca attacacaac aatcggcccc | ||
4081 tctgtaaaaa gccattccca ggctttgaaa tcgttcttga ccagcagtat cccaaagctg | ||
4141 tttataatta gcaaacgaat ttagatgggc ggaacttata ttggaactta cctgtaatgt | ||
4201 gaccaatttg tcgtcaacca caacgtcctt ggttaaaaaa tcagcaccga tggtagcttt | ||
4261 atattcgcga ctaaactttt gattgacgaa ctaaaatgac gatgttaaca aattgccaaa | ||
4321 gcaatactca tagagaagct gatgtaaaga tcgttaacca tatttgagct agtatttaat | ||
4381 aacaaagtga ataaatttta aaagcaatca ccttgtagcg acaaataaca acttatcgac | ||
4441 ataaaatcaa tgggaaattg cagtattgga ttttacagct caatacaaaa accaaaaaga | ||
4501 aaaatatact gaacgtataa aatttaacgc ttcaattg | ||
// |
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{"sequences":[{"id":3,"kind":"entity","sequence":{"type":"file","file_extension":"gb","file_content":"LOCUS first 21 bp DNA linear UNK 01-JAN-1980\nDEFINITION first.\nACCESSION first\nVERSION first\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 ttttacgata atgctccccc c\n//","overhang_crick_3prime":0,"overhang_watson_3prime":0}},{"id":5,"kind":"entity","sequence":{"type":"file","file_extension":"gb","file_content":"LOCUS second 12 bp DNA linear UNK 01-JAN-1980\nDEFINITION second.\nACCESSION second\nVERSION second\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 cccctcatgg gg\n//","overhang_crick_3prime":0,"overhang_watson_3prime":0}},{"id":7,"kind":"entity","sequence":{"type":"file","file_extension":"gb","file_content":"LOCUS third 13 bp DNA linear UNK 01-JAN-1980\nDEFINITION third.\nACCESSION third\nVERSION third\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 ggggatatat ttt\n//","overhang_crick_3prime":0,"overhang_watson_3prime":0}},{"id":9,"kind":"entity","sequence":{"type":"file","file_extension":"gb","file_content":"LOCUS name 38 bp DNA linear UNK 01-JAN-1980\nDEFINITION description.\nACCESSION id\nVERSION id\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 cccctcatgg ggggagcatt atcgtaaaat atatcccc\n//","overhang_crick_3prime":0,"overhang_watson_3prime":0}}],"sources":[{"id":1,"input":[],"output":3,"type":"file","kind":"source","info":{},"file_name":"gibson_assembly_fragments.fasta","file_format":"fasta","index_in_file":0},{"id":4,"input":[],"output":5,"type":"file","kind":"source","info":{},"file_name":"gibson_assembly_fragments.fasta","file_format":"fasta","index_in_file":1},{"id":6,"input":[],"output":7,"type":"file","kind":"source","info":{},"file_name":"gibson_assembly_fragments.fasta","file_format":"fasta","index_in_file":2},{"id":8,"input":[3,5,7],"output":9,"type":"gibson_assembly","kind":"source","info":{},"assembly":[[2,-1,"complement(9..12)","complement(1..4)"],[-1,-3,"complement(18..21)","complement(1..4)"]],"circular":false}],"description":"","primers":[{"id":1,"name":"fwd","sequence":"gatctcgccataaaagacag"},{"id":2,"name":"rvs","sequence":"ttaacaaagcgactataagt"}]} | ||
{ | ||
"sequences": [ | ||
{ "id": 3, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS first 21 bp DNA linear UNK 01-JAN-1980\nDEFINITION first.\nACCESSION first\nVERSION first\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 ttttacgata atgctccccc c\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 5, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS second 12 bp DNA linear UNK 01-JAN-1980\nDEFINITION second.\nACCESSION second\nVERSION second\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 cccctcatgg gg\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 7, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS third 13 bp DNA linear UNK 01-JAN-1980\nDEFINITION third.\nACCESSION third\nVERSION third\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 ggggatatat ttt\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 12, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS name 34 bp DNA circular UNK 01-JAN-1980\nDEFINITION description.\nACCESSION id\nVERSION id\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\nORIGIN\n 1 ttttacgata atgctccccc ctcatgggga tata\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } } | ||
], | ||
"sources": [ | ||
{ "id": 1, "input": [], "output": 3, "type": "file", "kind": "source", "info": {}, "file_name": "gibson_assembly_fragments.fasta", "file_format": "fasta", "index_in_file": 0 }, | ||
{ "id": 4, "input": [], "output": 5, "type": "file", "kind": "source", "info": {}, "file_name": "gibson_assembly_fragments.fasta", "file_format": "fasta", "index_in_file": 1 }, | ||
{ "id": 6, "input": [], "output": 7, "type": "file", "kind": "source", "info": {}, "file_name": "gibson_assembly_fragments.fasta", "file_format": "fasta", "index_in_file": 2 }, | ||
{ | ||
"id": 11, | ||
"input": [3, 5, 7], | ||
"output": 12, | ||
"type": "gibson_assembly", | ||
"kind": "source", | ||
"info": {}, | ||
"assembly": [ | ||
[1, 2, "18..21", "1..4"], | ||
[2, 3, "9..12", "1..4"], | ||
[3, 1, "10..13", "1..4"] | ||
], | ||
"circular": true | ||
} | ||
], | ||
"description": "", | ||
"primers": [ | ||
{ "id": 1, "name": "fwd", "sequence": "gatctcgccataaaagacag" }, | ||
{ "id": 2, "name": "rvs", "sequence": "ttaacaaagcgactataagt" } | ||
] | ||
} |
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{ | ||
"sequences": [ | ||
{ "id": 3, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS pj5_00001 27 bp DNA linear SYN 15-JAN-2024\nDEFINITION .\nACCESSION pj5_00001\nVERSION pj5_00001\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nCOMMENT teselagen_unique_id: 5adf735aa1811801e17d8aac\nFEATURES Location/Qualifiers\n misc_feature 10..17\n /label=\"A\"\nORIGIN\n 1 ggtctcaatt aaaaaattaa agagacc\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 5, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS pj5_00001 27 bp DNA linear SYN 15-JAN-2024\nDEFINITION .\nACCESSION pj5_00001\nVERSION pj5_00001\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nCOMMENT teselagen_unique_id: 5adf735aa1811801e17d8aac\nFEATURES Location/Qualifiers\n misc_feature complement(10..17)\n /label=\"B\"\nORIGIN\n 1 ggtctcatta acccccatat agagacc\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 7, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS pj5_00001 27 bp DNA linear SYN 15-JAN-2024\nDEFINITION .\nACCESSION pj5_00001\nVERSION pj5_00001\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nCOMMENT teselagen_unique_id: 5adf735aa1811801e17d8aac\nFEATURES Location/Qualifiers\n misc_feature 10..17\n /label=\"C\"\nORIGIN\n 1 ggtctcaata tgggggccgg agagacc\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 15, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS pj5_00001 35 bp DNA circular SYN 15-JAN-2024\nDEFINITION .\nACCESSION pj5_00001\nVERSION pj5_00001\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nCOMMENT teselagen_unique_id: 5adf735aa1811801e17d8aac\nFEATURES Location/Qualifiers\n misc_feature 11..25\n /label=\"VectorOUT\"\n misc_feature 32..36\n /label=\"VectorIN\"\nORIGIN\n 1 ttttattaag agaccttttt ggtctcaccg gtttt\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } }, | ||
{ "id": 19, "kind": "entity", "sequence": { "type": "file", "file_extension": "gb", "file_content": "LOCUS name 39 bp DNA circular UNK 01-JAN-1980\nDEFINITION description.\nACCESSION id\nVERSION id\nKEYWORDS .\nSOURCE .\n ORGANISM .\n .\nFEATURES Location/Qualifiers\n misc_feature 3..10\n /label=\"A\"\n misc_feature complement(12..19)\n /label=\"B\"\n misc_feature 21..28\n /label=\"C\"\n misc_feature 32..36\n /label=\"VectorIN\"\nORIGIN\n 1 attaaaaaat taacccccat atgggggccg gtttttttt\n//", "overhang_crick_3prime": 0, "overhang_watson_3prime": 0 } } | ||
], | ||
"sources": [ | ||
{ "id": 1, "input": [], "output": 3, "type": "file", "kind": "source", "info": {}, "file_name": "golden_gate.gb", "file_format": "genbank", "index_in_file": 0 }, | ||
{ "id": 4, "input": [], "output": 5, "type": "file", "kind": "source", "info": {}, "file_name": "golden_gate.gb", "file_format": "genbank", "index_in_file": 1 }, | ||
{ "id": 6, "input": [], "output": 7, "type": "file", "kind": "source", "info": {}, "file_name": "golden_gate.gb", "file_format": "genbank", "index_in_file": 2 }, | ||
{ "id": 14, "input": [], "output": 15, "type": "file", "kind": "source", "info": {}, "file_name": "golden_gate.gb", "file_format": "genbank", "index_in_file": 3 }, | ||
{ | ||
"id": 18, | ||
"input": [3, 5, 7, 15], | ||
"output": 19, | ||
"type": "restriction_and_ligation", | ||
"kind": "source", | ||
"info": {}, | ||
"assembly": [ | ||
[1, 2, "17..20", "8..11"], | ||
[2, 3, "17..20", "8..11"], | ||
[3, 4, "17..20", "28..31"], | ||
[4, 1, "5..8", "8..11"] | ||
], | ||
"circular": true, | ||
"restriction_enzymes": ["BsaI"] | ||
} | ||
], | ||
"description": "", | ||
"primers": [ | ||
{ "id": 1, "name": "fwd", "sequence": "gatctcgccataaaagacag" }, | ||
{ "id": 2, "name": "rvs", "sequence": "ttaacaaagcgactataagt" } | ||
] | ||
} |
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