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DOC: update paper
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Vini2 committed Sep 13, 2024
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Expand Up @@ -72,7 +72,7 @@ GraphBin-Tk can perform stand-alone metagenomic binning using MetaCoAG and bin r

## Binning, preparing binning results and bin refinement

GraphBin-Tk supports metagenome assemblies generated from three popular metagenome assemblers; metaSPAdes [@Nurk:2017] and MEGAHIT [@Li:2015] for short-read sequencing data and metaFlye [@Kolmogorov:2020] for long-read sequencing data. GraphBin-Tk can be launched using the command `gbintk`. A user can start the analysis by running the `metacoag` subcommand to bin a metagenomic dataset and obtain MAGs as shown in \autoref{fig1}. The inputs required are the contigs file, the assembly graph files and the read coverage of contigs. The read coverage of contigs can be obtained by running a coverage calculation tool such as Koverage [@Roach:2024]. The MetaCoAG binning result can be formatted using the `prepare` subcommand into a delimited text file such as `.csv` of `.tsv` that represents each contig and its bin name. This formatted binning result can be improved by providing to either GraphBin or GraphBin2 using the subcommands `graphbin` or `graphbin2` along with the contigs file, the assembly graph files and the read coverage of contigs (refer to \autoref{fig1}).
GraphBin-Tk supports metagenome assemblies generated from three popular metagenome assemblers; metaSPAdes [@Nurk:2017] and MEGAHIT [@Li:2015] for short-read sequencing data and metaFlye [@Kolmogorov:2020] for long-read sequencing data. GraphBin-Tk can be launched using the command `gbintk`. A user can start the analysis by running the `metacoag` subcommand to bin a metagenomic dataset and obtain MAGs as shown in \autoref{fig1}. The inputs required are the contigs file, the assembly graph files and the read coverage of contigs. The read coverage of contigs can be obtained by running a coverage calculation tool such as Koverage [@Roach:2024]. The MetaCoAG binning result can be formatted using the `prepare` subcommand into a delimited text file such as `.csv` or `.tsv` that represents each contig and its bin name. This formatted binning result can be improved by providing to either GraphBin or GraphBin2 using the subcommands `graphbin` or `graphbin2` with the contigs file, the assembly graph files and the read coverage of contigs (\autoref{fig1}).

## Visualisation

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