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MiXCR v4.4.2

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@github-actions github-actions released this 30 Jul 08:30

🚀 New features

  • Two-fold align step speedup for most of the protocol-specific presets (see the list below)
  • Import tags from sequence headers by parsing its content with regular expressions
  • Highly optimized generic presets for amplicon/rna-seq sequence by long-read sequencers such as Pacific Biosciences and Oxford Nanopore: generic-ont, generic-ont-with-umi, generic-pacbio, generic-pacbio-with-umi

🐞 Bug fixes

  • Fixes crush when input contains quality scores > 70
  • Fixes excessive memory consumption issue for long read data
  • Fix for crush in assemble with UMI tags but with consensus assembler turned off

👷 Other minor adjustments

  • Long-read J gene aligner optimization
  • FLAIRR-seq preset optimized with new long-read-optimized aligner
  • Quality trimming is disabled for long-read aligner
  • Removed qc reports for clustered alignments and clones
  • The following presets have been optimized by specifying a single reverse/direct alignment mode and now work faster:
    takara-human-rna-bcr-umi-smartseq, takara-human-rna-bcr-umi-smarter,takara-human-rna-tcr-umi-smartseq,takara-human-rna-tcr-umi-smarter-v2,takara-human-rna-tcr-smarter,takara-mouse-rna-bcr-smarter,takara-mouse-rna-tcr-smarter,10x-sc-xcr-vdj,10x-sc-5gex,abhelix-human-rna-xcr,bd-human-sc-xcr-rhapsody-cdr3,bd-mouse-sc-xcr-rhapsody-cdr3,bd-sc-xcr-rhapsody-full-length,cellecta-human-rna-xcr-umi-drivermap-air,illumina-human-rna-trb-ampliseq-sr,illumina-human-rna-trb-ampliseq-plus,irepertoire-human-rna-xcr-repseq-sr,irepertoire-human-rna-xcr-repseq-lr,irepertoire-mouse-rna-xcr-repseq-sr,irepertoire-mouse-rna-xcr-repseq-lr,irepertoire-human-rna-xcr-repseq-plus,irepertoire-mouse-rna-xcr-repseq-plus,irepertoire-human-dna-xcr-repseq-sr,irepertoire-human-dna-xcr-repseq-lr,milab-human-rna-ig-umi-multiplex,milab-human-rna-tcr-umi-race,milab-human-rna-tcr-umi-multiplex,milab-human-dna-tcr-multiplex,milab-human-dna-xcr-7genes-multiplex,milab-mouse-rna-tcr-umi-race,neb-human-rna-xcr-umi-nebnext,qiagen-human-rna-tcr-umi-qiaseq