Mandel Lab Protocols

Molecular Biology

• Gene Deletion in V. fischeri by allelic exchange
• Gibson Assembly
• dNTP stocks for PCR
• Semi-arbitrarily-primed PCR to identify transposon insertion sites

Protein

• Trichloroacetic acid (TCA) precipitation
• Bioinformatics Prediction Tools
• SDS-Page
• Immunoblotting

Microbiology

• Media and Buffers Recipes
  • Media
  • Buffers and Reagents
• Antibiotics
• Colony-Forming Units (CFU), Counting
• Conjugation
• Competition in vitro
• Electrocompetent Cells
• Glycerol stocks
• Luminescence reading in a single tube luminometer
• Transformation using tfoX induction
• Wrinkled Colony Assay

Lab Maintenance

• Preparing sterile supplies
• Shelf stocking

Squid

• Colonization
• Colonization for aggregates
• Colonization for INSeq
• Competition assay
• Fixation and staining

Computational

• Git and GitHub

Writing

• Style and Writing Guide

Equipment

• Autoclave
• Gel Doc
• QIAcube
• Plate Reader
• Leica EZ4 Steromicroscope
• Zeiss Axio Zoom V16 Microscope