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Bestie

wdl based hts-analysis for slurm cluster with enviroment modules.

Goals:

  • is to get generic alignment working.
  • basic variant calling (haplotypecallerGvcf).
  • somatic variant calling(MuTect2,...).
  • ichorCNA integration.
  • Variant annotation
  • Functional filtering of vcfs
  • Stability over > 10 samples
  • Rework dir structure to be more in line with warp/other public resources
  • End to end easybuild install

Relevant example resources for writing wdl files :

WARP BioWDL

How to use

Run the cromwell validation tool womtool to validate the input and generate a template input file. for sampleJson see ./tests/data/raw/fastq/samples.json. See below for the most simple run example

 java -Xmx8g -Dconfig.file=./path/to/cromwell.conf -jar ./path/to/cromwell.jar run Bestie.wdl -i inputs_integration.json

How to install

easybuild the required modules or use future wrapper module

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