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Modified graphrra.nf for Apptainer/Singularity.
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medmaca committed Nov 7, 2024
1 parent 9f0de4f commit 84a919a
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9 changes: 1 addition & 8 deletions modules/local/mageck/graphrra.nf
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Expand Up @@ -27,14 +27,7 @@ process MAGECK_GRAPHRRA {
#### Released under the MIT license. See git repository (https://github.com/nf-core/crisprseq) for full license text.
####
#### Orient a reference sequence according to reads orientation.
library(BiocFileCache)
bfc <- BiocFileCache("~/.cache/R/ExperimentHub")
library(ExperimentHub)
res <- bfcquery(bfc, "experimenthub.index.rds", field="rname", exact=TRUE)
bfcremove(bfc, rids=res\$rid)
eh = ExperimentHub()
Sys.setenv(BIOMART_CACHE=tempdir())
Sys.setenv(HOME = getwd()) # Required for Singularity/Apptainer
library(MAGeCKFlute)
library(ggplot2)
options(ggrepel.max.overlaps = Inf)
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