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update README with 2 diff options for bismark align
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sateeshperi committed Oct 22, 2024
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Expand Up @@ -30,20 +30,20 @@ On release, automated continuous integration tests run the pipeline on a full-si
The pipeline allows you to choose between running either [Bismark](https://github.com/FelixKrueger/Bismark) or [bwa-meth](https://github.com/brentp/bwa-meth) / [MethylDackel](https://github.com/dpryan79/methyldackel).
Choose between workflows by using `--aligner bismark` (default, uses bowtie2 for alignment), `--aligner bismark_hisat` or `--aligner bwameth`.

| Step | Bismark workflow | bwa-meth workflow |
| -------------------------------------------- | ---------------- | --------------------- |
| Generate Reference Genome Index _(optional)_ | Bismark | bwa-meth |
| Merge re-sequenced FastQ files | cat | cat |
| Raw data QC | FastQC | FastQC |
| Adapter sequence trimming | Trim Galore! | Trim Galore! |
| Align Reads | Bismark | bwa-meth |
| Deduplicate Alignments | Bismark | Picard MarkDuplicates |
| Extract methylation calls | Bismark | MethylDackel |
| Sample report | Bismark | - |
| Summary Report | Bismark | - |
| Alignment QC | Qualimap | Qualimap |
| Sample complexity | Preseq | Preseq |
| Project Report | MultiQC | MultiQC |
| Step | Bismark workflow | bwa-meth workflow |
| -------------------------------------------- | ------------------------ | --------------------- |
| Generate Reference Genome Index _(optional)_ | Bismark | bwa-meth |
| Merge re-sequenced FastQ files | cat | cat |
| Raw data QC | FastQC | FastQC |
| Adapter sequence trimming | Trim Galore! | Trim Galore! |
| Align Reads | Bismark (bowtie2/hisat2) | bwa-meth |
| Deduplicate Alignments | Bismark | Picard MarkDuplicates |
| Extract methylation calls | Bismark | MethylDackel |
| Sample report | Bismark | - |
| Summary Report | Bismark | - |
| Alignment QC | Qualimap | Qualimap |
| Sample complexity | Preseq | Preseq |
| Project Report | MultiQC | MultiQC |

## Usage

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