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Merge pull request #53 from PixelgenTechnologies/reading-the-docs
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Update README.md
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fbdtemme authored Jun 29, 2023
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Expand Up @@ -29,12 +29,12 @@ It takes a samplesheet as input and will process your data using `pixelator` to
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->

1. Build amplicon from input reads ([`pixelator concatenate`](https://github.com/PixelgenTechnologies/pixelator))
2. Read QC and filtering, correctness of PBS sequences ([`pixelator preqc | pixelator adapterqc`](https://github.com/PixelgenTechnologies/pixelator))
2. Read QC and filtering, correctness of the pixel binding sequence sequences ([`pixelator preqc | pixelator adapterqc`](https://github.com/PixelgenTechnologies/pixelator))
3. Assign a marker (barcode) to each read ([`pixelator demux`](https://github.com/PixelgenTechnologies/pixelator))
4. Error correction, duplicate removal, compute read counts ([`pixelator collapse`](https://github.com/PixelgenTechnologies/pixelator))
5. Compute the components/clusters of the graph from the edge list matrix.([`pixelator graph`](https://github.com/PixelgenTechnologies/pixelator))
6. Filter, annotate and call cells on samples ([`pixelator annotate`](https://github.com/PixelgenTechnologies/pixelator))
7. Analyze components/clusters of the graph.([`pixelator analysis`](https://github.com/PixelgenTechnologies/pixelator))
5. Compute the components of the graph from the edge list in order to create putative cells ([`pixelator graph`](https://github.com/PixelgenTechnologies/pixelator))
6. Call and annotate cells ([`pixelator annotate`](https://github.com/PixelgenTechnologies/pixelator))
7. Analyze the cells for polarization and colocalization ([`pixelator analysis`](https://github.com/PixelgenTechnologies/pixelator))
8. Report generation ([`pixelator report`](https://github.com/PixelgenTechnologies/pixelator))

## Usage
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```csv
sample,design,panel,fastq_1,fastq_2
uropod_control,D21,UNO_D21_conjV21.csv,uropod_control_300k_S1_R1_001.fastq.gz,uropod_control_300k_S1_R2_001.fastq.gz
uropod_control,D21,human-sc-immunology-spatial-proteomics,uropod_control_300k_S1_R1_001.fastq.gz,uropod_control_300k_S1_R2_001.fastq.gz
```

Each row represents a sample and gives the design, a panel file and the input fastq files.
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