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Important! Template update for nf-core/tools v3.2.0.dev0 #102

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4 changes: 4 additions & 0 deletions .editorconfig
Original file line number Diff line number Diff line change
Expand Up @@ -31,3 +31,7 @@ indent_size = unset
# ignore python and markdown
[*.{py,md}]
indent_style = unset

# ignore ro-crate metadata files
[**/ro-crate-metadata.json]
insert_final_newline = unset
1 change: 0 additions & 1 deletion .github/ISSUE_TEMPLATE/bug_report.yml
Original file line number Diff line number Diff line change
Expand Up @@ -9,7 +9,6 @@ body:

- [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
- [nf-core/testpipeline pipeline documentation](https://nf-co.re/testpipeline/usage)

- type: textarea
id: description
attributes:
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15 changes: 13 additions & 2 deletions .github/workflows/awsfulltest.yml
Original file line number Diff line number Diff line change
Expand Up @@ -19,19 +19,30 @@ jobs:
if: github.repository == 'nf-core/testpipeline' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch'
runs-on: ubuntu-latest
steps:
- uses: octokit/request-action@v2.x
- name: Get PR reviews
uses: octokit/request-action@v2.x
if: github.event_name != 'workflow_dispatch'
id: check_approvals
continue-on-error: true
with:
route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews?per_page=100
env:
GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}
- id: test_variables

- name: Check for approvals
if: ${{ failure() && github.event_name != 'workflow_dispatch' }}
run: |
echo "No review approvals found. At least 2 approvals are required to run this action automatically."
exit 1

- name: Check for enough approvals (>=2)
id: test_variables
if: github.event_name != 'workflow_dispatch'
run: |
JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}'
CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length')
test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required

- name: Launch workflow via Seqera Platform
uses: seqeralabs/action-tower-launch@v2
# TODO nf-core: You can customise AWS full pipeline tests as required
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2 changes: 2 additions & 0 deletions .github/workflows/ci.yml
Original file line number Diff line number Diff line change
Expand Up @@ -46,6 +46,8 @@ jobs:
steps:
- name: Check out pipeline code
uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4
with:
fetch-depth: 0

- name: Set up Nextflow
uses: nf-core/setup-nextflow@v2
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51 changes: 32 additions & 19 deletions .github/workflows/download_pipeline.yml
Original file line number Diff line number Diff line change
Expand Up @@ -28,8 +28,23 @@ env:
NXF_ANSI_LOG: false

jobs:
configure:
runs-on: ubuntu-latest
outputs:
REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }}
REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }}
REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }}
steps:
- name: Get the repository name and current branch
id: get_repo_properties
run: |
echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT"
echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT"
echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT"

download:
runs-on: ubuntu-latest
needs: configure
steps:
- name: Install Nextflow
uses: nf-core/setup-nextflow@v2
Expand All @@ -52,12 +67,6 @@ jobs:
python -m pip install --upgrade pip
pip install git+https://github.com/nf-core/tools.git@dev

- name: Get the repository name and current branch set as environment variable
run: |
echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV}
echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV}
echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV}

- name: Make a cache directory for the container images
run: |
mkdir -p ./singularity_container_images
Expand All @@ -66,55 +75,59 @@ jobs:
env:
NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
run: |
nf-core pipelines download ${{ env.REPO_LOWERCASE }} \
--revision ${{ env.REPO_BRANCH }} \
--outdir ./${{ env.REPOTITLE_LOWERCASE }} \
nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \
--revision ${{ needs.configure.outputs.REPO_BRANCH }} \
--outdir ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} \
--compress "none" \
--container-system 'singularity' \
--container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" \
--container-cache-utilisation 'amend' \
--download-configuration 'yes'

- name: Inspect download
run: tree ./${{ env.REPOTITLE_LOWERCASE }}
run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}

- name: Inspect container images
run: tree ./singularity_container_images | tee ./container_initial

- name: Count the downloaded number of container images
id: count_initial
run: |
image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
echo "Initial container image count: $image_count"
echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV}
echo "IMAGE_COUNT_INITIAL=$image_count" >> "$GITHUB_OUTPUT"

- name: Run the downloaded pipeline (stub)
id: stub_run_pipeline
continue-on-error: true
env:
NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
NXF_SINGULARITY_HOME_MOUNT: true
run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
run: nextflow run ./${{needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results
- name: Run the downloaded pipeline (stub run not supported)
id: run_pipeline
if: ${{ job.steps.stub_run_pipeline.status == failure() }}
if: ${{ steps.stub_run_pipeline.outcome == 'failure' }}
env:
NXF_SINGULARITY_CACHEDIR: ./singularity_container_images
NXF_SINGULARITY_HOME_MOUNT: true
run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results
run: nextflow run ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -profile test,singularity --outdir ./results

- name: Count the downloaded number of container images
id: count_afterwards
run: |
image_count=$(ls -1 ./singularity_container_images | wc -l | xargs)
echo "Post-pipeline run container image count: $image_count"
echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV}
echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT"

- name: Compare container image counts
run: |
if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then
initial_count=${{ env.IMAGE_COUNT_INITIAL }}
final_count=${{ env.IMAGE_COUNT_AFTER }}
if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then
initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}
final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}
difference=$((final_count - initial_count))
echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!"
tree ./singularity_container_images
tree ./singularity_container_images > ./container_afterwards
diff ./container_initial ./container_afterwards
exit 1
else
echo "The pipeline can be downloaded successfully!"
Expand Down
2 changes: 1 addition & 1 deletion .github/workflows/linting_comment.yml
Original file line number Diff line number Diff line change
Expand Up @@ -11,7 +11,7 @@ jobs:
runs-on: ubuntu-latest
steps:
- name: Download lint results
uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7
uses: dawidd6/action-download-artifact@20319c5641d495c8a52e688b7dc5fada6c3a9fbc # v8
with:
workflow: linting.yml
workflow_conclusion: completed
Expand Down
33 changes: 0 additions & 33 deletions .github/workflows/release-announcements.yml
Original file line number Diff line number Diff line change
Expand Up @@ -27,39 +27,6 @@ jobs:

${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics

send-tweet:
runs-on: ubuntu-latest

steps:
- uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5
with:
python-version: "3.10"
- name: Install dependencies
run: pip install tweepy==4.14.0
- name: Send tweet
shell: python
run: |
import os
import tweepy

client = tweepy.Client(
access_token=os.getenv("TWITTER_ACCESS_TOKEN"),
access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"),
consumer_key=os.getenv("TWITTER_CONSUMER_KEY"),
consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"),
)
tweet = os.getenv("TWEET")
client.create_tweet(text=tweet)
env:
TWEET: |
Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}!

Please see the changelog: ${{ github.event.release.html_url }}
TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }}
TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }}
TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }}
TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }}

bsky-post:
runs-on: ubuntu-latest
steps:
Expand Down
2 changes: 1 addition & 1 deletion .gitpod.yml
Original file line number Diff line number Diff line change
Expand Up @@ -7,4 +7,4 @@ tasks:

vscode:
extensions:
#- nf-core.nf-core-extensionpack # https://github.com/nf-core/vscode-extensionpack
- nf-core.nf-core-extensionpack # https://github.com/nf-core/vscode-extensionpack
2 changes: 1 addition & 1 deletion .nf-core.yml
Original file line number Diff line number Diff line change
@@ -1,7 +1,7 @@
lint:
files_unchanged:
- .github/workflows/linting.yml
nf_core_version: 3.1.0
nf_core_version: 3.2.0.dev0
repository_type: pipeline
template:
author: nf-core
Expand Down
2 changes: 1 addition & 1 deletion .pre-commit-config.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -7,7 +7,7 @@ repos:
- prettier@3.2.5

- repo: https://github.com/editorconfig-checker/editorconfig-checker.python
rev: "3.0.3"
rev: "3.1.2"
hooks:
- id: editorconfig-checker
alias: ec
1 change: 1 addition & 0 deletions .prettierignore
Original file line number Diff line number Diff line change
Expand Up @@ -10,3 +10,4 @@ testing/
testing*
*.pyc
bin/
ro-crate-metadata.json
2 changes: 1 addition & 1 deletion LICENSE
Original file line number Diff line number Diff line change
@@ -1,6 +1,6 @@
MIT License

Copyright (c) nf-core
Copyright (c) The nf-core/testpipeline team

Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
Expand Down
5 changes: 1 addition & 4 deletions README.md
Original file line number Diff line number Diff line change
Expand Up @@ -29,10 +29,7 @@

<!-- TODO nf-core: Include a figure that guides the user through the major workflow steps. Many nf-core
workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. -->
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->

1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))

## Usage

Expand Down
4 changes: 1 addition & 3 deletions conf/test.config
Original file line number Diff line number Diff line change
Expand Up @@ -25,8 +25,6 @@ params {
// Input data
// TODO nf-core: Specify the paths to your test data on nf-core/test-datasets
// TODO nf-core: Give any required params for the test so that command line flags are not needed
input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'

// Genome references
input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'// Genome references
genome = 'R64-1-1'
}
1 change: 0 additions & 1 deletion docs/output.md
Original file line number Diff line number Diff line change
Expand Up @@ -44,7 +44,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
[MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory.

Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see <http://multiqc.info>.

### Pipeline information

<details markdown="1">
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2 changes: 1 addition & 1 deletion docs/usage.md
Original file line number Diff line number Diff line change
Expand Up @@ -130,7 +130,7 @@ Several generic profiles are bundled with the pipeline which instruct the pipeli
> [!IMPORTANT]
> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported.

The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is suported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).
The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is supported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation).

Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important!
They are loaded in sequence, so later profiles can overwrite earlier profiles.
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4 changes: 2 additions & 2 deletions modules.json
Original file line number Diff line number Diff line change
Expand Up @@ -7,12 +7,12 @@
"nf-core": {
"fastqc": {
"branch": "master",
"git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164",
"git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296",
"installed_by": ["modules"]
},
"multiqc": {
"branch": "master",
"git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
"git_sha": "f0719ae309075ae4a291533883847c3f7c441dad",
"installed_by": ["modules"]
}
}
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20 changes: 10 additions & 10 deletions modules/nf-core/fastqc/main.nf

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2 changes: 0 additions & 2 deletions modules/nf-core/fastqc/tests/tags.yml

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2 changes: 1 addition & 1 deletion modules/nf-core/multiqc/environment.yml

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4 changes: 2 additions & 2 deletions modules/nf-core/multiqc/main.nf

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