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primer_matching

An R script to find sequencing primers useful to the plasmid at hand

Nicholas McGlincy, 27th July 2013, Baltimore MD, USA

Requires R, and a version advanced enough to run the Biostrings package. I used Biostrings because of the convenient functions and classes, but loading it slows the script down to ~5 seconds

Usage from the terminal window:

Rscript primerMatching.R [plasmid file] [primer file]

Plasmid file must be in fasta format. Primer file is a .csv download of the group sequencing primer gsheet.

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