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combine error #503

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seasky002002 opened this issue Jan 28, 2022 · 2 comments
Open

combine error #503

seasky002002 opened this issue Jan 28, 2022 · 2 comments

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@seasky002002
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the program hangup when running combine bams, here are the logs,
Thu Jan 27 20:11:41 CST 2022
Combine R1/R2 alignment files ...
Logs: logs/rep1/mapping_combine.log
make: *** [/usr/local/bin/HiC-Pro_2.11.4/bin/../scripts//Makefile:115: bowtie_com
bine] Error 129
Hangup

And the mapping_combine.log only has two lines:
more result/logs/rep1/mapping_combine.log
/usr/local/anaconda/bin/samtools merge -@ 2 -n -f bowtie_results/bwt2/rep1/SRR401
5027_pass_2_hg19.bwt2merged.bam bowtie_results/bwt2_global/rep1/SRR4015027_pass_2
_hg19.bwt2glob.bam bowtie_results/bwt2_local/rep1/SRR4015027_pass_2_hg19.bwt2glob
.unmap_bwt2loc.bam
/usr/local/anaconda/bin/samtools merge -@ 2 -n -f bowtie_results/bwt2/rep1/SRR401
5027_pass_1_hg19.bwt2merged.bam bowtie_results/bwt2_global/rep1/SRR4015027_pass_1
_hg19.bwt2glob.bam bowtie_results/bwt2_local/rep1/SRR4015027_pass_1_hg19.bwt2glob
.unmap_bwt2loc.bam

Would you please help me to debug?
Thank you!

@nservant
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nservant commented Feb 3, 2022

Do you have the bwt2merged.bam files ? otherwise, my feeling is that you had a memory issue with the samtools merge command.

@seasky002002
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seasky002002 commented Feb 3, 2022 via email

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