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qiita_pet/support_files/doc/source/faq.rst

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`this 3 minute read about Markdown <https://guides.github.com/features/mastering-markdown/>`__.
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I want to transfer a lot of files to Qiita, is there an easy way?
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Yes! This is available in the "Upload Files" section of each study by accessing the tab
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"Upload via Remote Server (ADVANCED)".
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We currently suggest using scp, note that your server where you store your files should allow
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connecting to it via scp - in other words, this only works when moving files from a server to Qiita.
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Now, the way it works is that you need to create a new secure key to that server, imagine
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that you are making a copy of your storage-shed key, then you share that key to Qiita (you will give
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access to Qiita to run a single copy command in your server), Qiita uses that key and securely
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destroy it.
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To take advantage of this feature you need to:
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#. Prepare all the files you want to transfer in a single folder (or you can transfer
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multiple times from multiple folders into one study). Note that Qiita will only collect
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files with valid extensions from the top level directory (no sub-directories); see the
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top of the "Upload Files" page within your study for the latests list of valid extensions.
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#. In your server (this needs to be run within your home directory in the server where you
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store the files!), generate a new key by running:
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`ssh-keygen -t rsa -C "ssh test key" -f ~/.ssh/qiita-key -P ""`. Here is where you are
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creating that key to your storage-shed.
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#. Allow access using the new key to new connections (this also needs to be run in the
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remote server): `cat ~/.ssh/qiita-key.pub >> ~/.ssh/authorized_keys`. This tells the
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server that is OK to give access to the key created to your storage-shed; note that if
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you want to completely stop that key to work you can open that file and remove the line
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with the name of this key.
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#. Dowload your new generated key `qiita-key` (the file) to your local computer and use it
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in the `Key` option of "Upload via Remote Server (ADVANCED)".
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Using this key you can `List Files` to test the connection and verify the list of study files. Then,
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if the connection is made and files are correct, press 'Transfer Files' to initiate the transfer.
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Note that if you click multiple times, too quickly there is a chance that your server will block
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Qiita, if this happens, just wait a few minutes and retry again.
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How do I update the sample or preparation file?
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-----------------------------------------------
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Remember, these are separate files so they need to be updated separately. In both cases,
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the easiest is to upload the new file from your computer to Qiita using the `Upload Files`
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button in your study. Once the file you want to use is there you can use them within Qiita.
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To update a sample information file: Click on `Sample Information` button in the your study
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page, then use the `Update sample information` section on that page to select your file and
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update it. Note that for this you can also directly upload your file via the
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`Direct upload file (< 2MB)`.
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To update a preparation information file: Click on the preparation you want to update within
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your study page, then click on `Summary`, and use the `Update prep information` section on
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that page to select your file and update it.
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Note that these information is generally independent of the sequence processing so you
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don't need to reprocess your sequences; however, if you use your study in an analysis, you will
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need to recreate that analysis to use the updated sample or preparation metadata
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When do I need the run_prefix in my preparation information file?
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It depends on your sequence processing but in general it will facilitate loading your
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files to your preparation in Qiita.
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First of all, this is a prefix value so you only need the beginning of the file
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name to load the files in Qiita; for example if your file names for a given sample are:
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AWERWADFA_I1.fastq.gz, AWERWADFA_R1.fastq.gz, AWERWADFA_R2.fastq.gz, the run_prefix for
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the sample should be AWERWADFA. Qiita will use that to group those files under the same
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sample with that run_prefix.
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Now the run_prefix is used constantly within file selection and processing in all file types but
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specially on:
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- BIOM: the run_prefix is used to rename the samples in your BIOM to match the sample names
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in Qiita. Basically, if you add the sample names in your BIOM file as the run_prefix and the
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sample name in Qiita in the sample_name column of your preparation, Qiita will automatically
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rename them to match.
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- per sample FASTQ: run_prefix is the way to link which sample goes with which files so using here
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will facilitate loading your files to the preparation and then used for processing, without it
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Qiita will not be able to process your samples.
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How to cite Qiita?
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