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trimming beyond adapter? #34

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RichardCorbett opened this issue Apr 25, 2016 · 1 comment
Open

trimming beyond adapter? #34

RichardCorbett opened this issue Apr 25, 2016 · 1 comment

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@RichardCorbett
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Hey There.
I've been using your tool quite a bit lately. It has been the best option for adapter trimming in my hands.

I've just been handed some illumina reads which are much longer than the insert, so I expect to see quite a lot of adapter.

I'm wondering if skewer will work if I can only provide the first 50 bases of the adapter sequence but the read contains more adapter sequence after what I provided. For example. my read is 200 bases, and there is only a 50 base insert which means I'll be getting an extra 150bp off the end of my insert. If I only know the first 50 bases of the adapter/primer sequencer, will the extra 100bp after my provided adapter sequence still be trimmed, or will no trimming occur?

thanks,
Richard

@ATpoint
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ATpoint commented Sep 18, 2016

After a quick test with a dummy fastq file, with only the sequence:
ACCTTNTCCCAAGCAAATGGCAAACATAATAAAGGGTATAATCCCATAGGAGGAT
and ./skewer -x TGGCAAAC -l 1, I get
ACCTTNTCCCAAGCAAA.
So everything downstream of the adapter seems to be removed.

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