- version 07082022
- tested on conda v4.12.0
- optional primer site based filter
- report: includes statistics for the FASTA files and number of known and novel transcripts
- edits to the transcript file output for compatibility with IsoMix
All the sample files (pass-reads only) should be in FASTA format (.fa)
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Downloading the workflow with
git clone
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Downloading relevant reference file and creating a conda environment
cd discoAnt
bash discoAnt_setup.sh
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Running a test script to check installation
conda activate discoAnt
bash discoAnt_v2_SIRV_test.sh
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Update discoAnt_params.txt with gene info and paths to relevant folders before running the workflow with the data set of your choice
conda activate discoAnt.env
bash discoAnt_v2.1.sh