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Merge pull request #65 from smithlabcode/update-adaptor-contaminants
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Updating adapter and contaminant files from FastQC v0.12.0
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andrewdavidsmith authored Aug 9, 2024
2 parents 5d29eec + 1601cbb commit cac5bcc
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53 changes: 27 additions & 26 deletions Configuration/adapter_list.txt
Original file line number Diff line number Diff line change
@@ -1,26 +1,27 @@
# This file contains a set of sequence fragments which will be explicitly
# searched against your library. The reporting will be similar to the
# Kmer plot, except that every sequence in this list will be shown so
# you can use this to judge the level of adapter read-through even if those
# adapter sequences aren't picked out by the Kmer module.
#
# Since every sequence here will be analysed and the results plotted it
# doesn't make any sense to include duplicate sequences, or to add too
# many sequences since your plot will end up a mess.
#
# You can add more sequences to the file by putting one line per entry
# and specifying a name[tab]sequence. If the contaminant you add is
# likely to be of use to others please consider sending it to the FastQ
# authors, either via a bug report at www.bioinformatics.babraham.ac.uk/bugzilla/
# or by directly emailing simon.andrews@babraham.ac.uk so other users of
# the program can benefit.
#
# For the time being it's going to be easier to interpret this plot if all
# of the sequences provided are the same length, so we've gone with 12bp
# fragments for now.

Illumina Universal Adapter AGATCGGAAGAG
Illumina Small RNA 3 prime Adapter TGGAATTCTCGG
Illumina Small RNA 5 prime Adapter GATCGTCGGACT
Nextera Transposase Sequence CTGTCTCTTATA
SOLID Small RNA Adapter CGCCTTGGCCGT
# This file contains a set of sequence fragments which will be explicitly
# searched against your library. The reporting will be similar to the
# Kmer plot, except that every sequence in this list will be shown so
# you can use this to judge the level of adapter read-through even if those
# adapter sequences aren't picked out by the Kmer module.
#
# Since every sequence here will be analysed and the results plotted it
# doesn't make any sense to include duplicate sequences, or to add too
# many sequences since your plot will end up a mess.
#
# You can add more sequences to the file by putting one line per entry
# and specifying a name[tab]sequence. If the contaminant you add is
# likely to be of use to others please consider sending it to the FastQ
# authors, either via a bug report at www.bioinformatics.babraham.ac.uk/bugzilla/
# or by directly emailing simon.andrews@babraham.ac.uk so other users of
# the program can benefit.
#
# For the time being it's going to be easier to interpret this plot if all
# of the sequences provided are the same length, so we've gone with 12bp
# fragments for now.

Illumina Universal Adapter AGATCGGAAGAG
Illumina Small RNA 3' Adapter TGGAATTCTCGG
Illumina Small RNA 5' Adapter GATCGTCGGACT
Nextera Transposase Sequence CTGTCTCTTATA
PolyA AAAAAAAAAAAA
PolyG GGGGGGGGGGGG
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