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Merge pull request #65 from smithlabcode/update-adaptor-contaminants
Updating adapter and contaminant files from FastQC v0.12.0
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Original file line number | Diff line number | Diff line change |
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@@ -1,26 +1,27 @@ | ||
# This file contains a set of sequence fragments which will be explicitly | ||
# searched against your library. The reporting will be similar to the | ||
# Kmer plot, except that every sequence in this list will be shown so | ||
# you can use this to judge the level of adapter read-through even if those | ||
# adapter sequences aren't picked out by the Kmer module. | ||
# | ||
# Since every sequence here will be analysed and the results plotted it | ||
# doesn't make any sense to include duplicate sequences, or to add too | ||
# many sequences since your plot will end up a mess. | ||
# | ||
# You can add more sequences to the file by putting one line per entry | ||
# and specifying a name[tab]sequence. If the contaminant you add is | ||
# likely to be of use to others please consider sending it to the FastQ | ||
# authors, either via a bug report at www.bioinformatics.babraham.ac.uk/bugzilla/ | ||
# or by directly emailing simon.andrews@babraham.ac.uk so other users of | ||
# the program can benefit. | ||
# | ||
# For the time being it's going to be easier to interpret this plot if all | ||
# of the sequences provided are the same length, so we've gone with 12bp | ||
# fragments for now. | ||
|
||
Illumina Universal Adapter AGATCGGAAGAG | ||
Illumina Small RNA 3 prime Adapter TGGAATTCTCGG | ||
Illumina Small RNA 5 prime Adapter GATCGTCGGACT | ||
Nextera Transposase Sequence CTGTCTCTTATA | ||
SOLID Small RNA Adapter CGCCTTGGCCGT | ||
# This file contains a set of sequence fragments which will be explicitly | ||
# searched against your library. The reporting will be similar to the | ||
# Kmer plot, except that every sequence in this list will be shown so | ||
# you can use this to judge the level of adapter read-through even if those | ||
# adapter sequences aren't picked out by the Kmer module. | ||
# | ||
# Since every sequence here will be analysed and the results plotted it | ||
# doesn't make any sense to include duplicate sequences, or to add too | ||
# many sequences since your plot will end up a mess. | ||
# | ||
# You can add more sequences to the file by putting one line per entry | ||
# and specifying a name[tab]sequence. If the contaminant you add is | ||
# likely to be of use to others please consider sending it to the FastQ | ||
# authors, either via a bug report at www.bioinformatics.babraham.ac.uk/bugzilla/ | ||
# or by directly emailing simon.andrews@babraham.ac.uk so other users of | ||
# the program can benefit. | ||
# | ||
# For the time being it's going to be easier to interpret this plot if all | ||
# of the sequences provided are the same length, so we've gone with 12bp | ||
# fragments for now. | ||
|
||
Illumina Universal Adapter AGATCGGAAGAG | ||
Illumina Small RNA 3' Adapter TGGAATTCTCGG | ||
Illumina Small RNA 5' Adapter GATCGTCGGACT | ||
Nextera Transposase Sequence CTGTCTCTTATA | ||
PolyA AAAAAAAAAAAA | ||
PolyG GGGGGGGGGGGG |
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