dsRIP-Seq is an R notebook containing an example analysis for identifying and comparing double stranded RNAs (dsRNAs) from dsRIP-Seq experiments. dsRNAs are abundantly present in cells and play multiple regulatory functions. dsRNAs of viral origin activate innate immune responses. Since RNA editing and modifications affect the structure and recognition of RNAs, their alteration can result in the accumulation of aberrant endogenous dsRNAs inducing a deleterious innate immune response.
The dataset of worked example analyzed in dsRIP-Seq was generated in the following publication:
Gao Y, Vasic R, Song Y, Teng R, Liu C, Gbyli R, Biancon G, Nelakanti R, Lobben K, Kudo E, Liu W, Ardasheva A, Fu X, Wang X, Joshi P, Lee V, Dura B, Viero G, Iwasaki A, Fan R, Xiao A, Flavell RA, Li HB, Tebaldi T, Halene S. m6A Modification Prevents Formation of Endogenous Double-Stranded RNAs and Deleterious Innate Immune Responses during Hematopoietic Development. Immunity. 2020 Jun 16;52(6):1007-1021.e8.
